Adipocyte Death, Adipose Tissue Remodeling and Obesity Complications

Abstract

OBJECTIVE- To determine the role of adipocyte death in obesity-induced adipose tissue (AT) inflammation and obesity complications.

RESEARCH DESIGN AND METHODS- Male C57BL/6 mice were fed a high fat diet for 20 weeks to induce obesity. Every four weeks, insulin resistance (IR) was assessed by intraperitoneal insulin tolerance tests (ITT), and epididymal and inguinal subcutaneous AT (eAT, iAT) and livers were harvested for histological, immunohistochemical and gene expression analyses.

RESULTS- Frequency of adipocyte death in eAT increased from <0.1% at baseline to 16% at week 12 coincident with increases in 1) depot weight, 2) AT macrophages (ATMΦs) expressing F4/80 and CD11c, 3) mRNA for tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1) and interleukin-10, and 4) IR. ATMΦs in crown-like structures surrounding dead adipocytes expressed TNF-α and interleukin-6 proteins. Adipocyte number began to decline at week 12. At week 16 adipocyte death reached ∼80%, coincident with maximal expression of CD11c and inflammatory genes, loss (40%) of eAT mass, widespread collagen deposition and accelerated hepatic macrosteatosis. By week 20 adipocyte number was restored with small adipocytes, coincident with reduced adipocyte death (4-fold), CD11c and MCP-1 gene expression (2-fold), and IR (35%). eAT weight did not increase at week 20 and was inversely correlated with liver weight after week 12 (r = -0. 85, P < 0.001). In iAT adipocyte death was first detected at week 12 and remained ≤ 3%.

CONCLUSIONS- These results implicate depot-selective adipocyte death and MΦ-mediated AT remodeling in inflammatory and metabolic complications of murine obesity.