Abstract

Objective: We sought to evaluate the entire picture of all monocyte chemotactic factors that potentially contribute to adipose tissue macrophage accumulation in obesity.

Research Design and Methods: Expression and regulation of members in the entire chemokine superfamily were evaluated in adipose tissue and isolated adipocytes of obese versus lean mice. Kinetics of adipose tissue macrophage infiltration was characterized by FACS. The effects of fatty acids on stimulation of chemokine expression in adipocytes and underlying mechanisms were investigated.

Results: Six monocyte chemotactic factors were found to be predominantly upregulated in isolated adipocytes vs. stromal vascular cells in obese mice for the first time though most of them were previously reported to be upregulated in whole adipose tissue. In diet-induced obese mice, adipose tissue enlargement, increase of adipocyte number, and elevation of multiple chemokine expression precede the initiation of macrophage infiltration. Free fatty acids are found to be inducers for upregulating these chemokines in 3T3-L1 adipocytes and this effect can be partially blunted by reducing TLR4 expression. FFAs induce expression of monocyte chemotactic factors in adipocytes via both transcription dependent and independent mechanisms. In contrast to reported role of JNK as the exclusive mediator of FFAs-induced MCP-1 expression in macrophages, we show a novel role of IKKβ in mediating FFAs-induced upregulation of all six chemokines and a role of JNK in FFAs-induced upregulation of MCP-1 and MCP-3.

Conclusions: Multiple chemokines derived from adipocytes might contribute to obesity-related WAT macrophage infiltration with FFAs as potential triggers and involvement of both IKKβ and JNK.