A2B adenosine receptors prevent insulin resistance by inhibiting adipose tissue inflammation via maintaining alternative macrophage activation
- Balázs Csóka1⇑,
- Balázs Koscsó1,
- Gábor Törő2,
- Endre Kókai2,
- László Virág2,3,
- Zoltán H. Németh1,4,
- Pál Pacher5,
- Péter Bai2,3 and
- György Haskó1,2⇑
- 1Department of Surgery, Rutgers New Jersey Medical School, 185 South Orange Ave, Newark, NJ 07103, USA
- 2Department of Medical Chemistry, Medical and Health Science Center, University of Debrecen, Egyetem Tér 1., H-4032 Debrecen, Hungary
- 3Cell Biology and Signalling Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary
- 4Department of Surgery, Morristown Memorial Hospital, Morristown, NJ 07960, USA
- 5National Institute on Alcohol Abuse and Alcoholism, 12420 Parklawn Dr., MSC-8115, Bethesda, MD 20892-8115, USA
- Corresponding authors: Balázs Csóka, E-mail: , and György Haskó, E-mail:
Obesity causes increased classical and decreased alternative macrophage activation, which in turn cause insulin resistance in target organs. Because A2B adenosine receptors (AR)s are important regulators of macrophage activation, we examined the role of A2BARs in adipose tissue inflammation and insulin resistance. A2BAR deletion impaired glucose and lipid metabolism in mice fed chow but not a high fat diet, which was paralleled by dysregulation of the adipokine system and increased classical macrophage activation and inhibited alternative macrophage activation. The expression of alternative macrophage activation-specific transcriptions factors, including CCAAT/enhancer binding protein β, interferon regulatory factor 4, and peroxisome proliferator-activated receptor γ was decreased in A2BAR deficient mice. Furthermore, in in vitro studies, we found that stimulation of A2BARs suppressed free fatty acid-induced deleterious inflammatory and metabolic activation of macrophages. Moreover, AR activation upregulated the interleukin-4-induced expression of CCAAT/enhancer binding protein β, interferon regulatory factor 4, and peroxisome proliferator-activated receptor γ in macrophages. Altogether, our results indicate that therapeutic strategies targeting A2BARs hold promise for preventing adipose tissue inflammation and insulin resistance.
- Received April 10, 2013.
- Accepted October 31, 2013.
- © 2013 by the American Diabetes Association.
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