Identification and characterisation of glucagon-like peptide-1 receptor expressing cells using a new transgenic mouse model
- Paul Richards*,1,
- Helen E Parker*,1,
- Alice E Adriaenssens1,
- Joshua M Hodgson1,
- Simon C Cork2,
- Stefan Trapp2,
- Fiona M Gribble†,1⇑ and
- Frank Reimann†,1
- 1Cambridge Institute for Medical Research and MRC Metabolic Diseases Unit, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0XY, UK
- 2Department of Neuroscience, Physiology and Pharmacology, University College London, London WC1E 6BT, UK
- Corresponding author: Fiona M Gribble Email:
Glucagon-like peptide-1 (GLP-1) is an intestinal hormone with widespread actions on metabolism. Therapies based on GLP-1 are highly effective because they increase glucose-dependent insulin secretion in people with type 2 diabetes, but many reports suggest that GLP-1 has additional beneficial, or in some cases potentially dangerous, actions on other tissues, including the heart, vasculature, exocrine pancreas, liver and central nervous system. Identifying which tissues express the GLP-1 receptor (GLP1R) is critical for the development of GLP-1 based therapies. Our objective was to identify and characterise the targets of GLP-1 in mice, using a method independent of GLP1R antibodies. Using newly-generated glp1r-cre mice crossed with fluorescent reporter strains, we show that major sites of glp1r expression include pancreatic β and δ-cells, vascular smooth muscle, cardiac atrium, gastric antrum/pylorus, enteric neurones and vagal and dorsal root ganglia. In the central nervous sytem, glp1r-fluorescent cells were abundant in the area postrema, arcuate nucleus, paraventricular nucleus and ventromedial hypothalamus. Sporadic glp1r-fluorescent cells were found in pancreatic ducts. No glp1r-fluorescence was observed in ventricular cardiomyocytes. Glp1r-positive enteric and vagal neurons were activated by GLP-1, and may contribute to intestinal and central responses to locally-released GLP-1, such as regulation of intestinal secretomotor activity and appetite.
* Joint first authors
† Joint senior authors
- Received September 19, 2013.
- Accepted November 21, 2013.
- © 2013 by the American Diabetes Association.
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