Metabolic-surgery improves insulin-resistance and type-2 diabetes, possibly related to weight-loss. We have performed a novel sleeve-gastrectomy in rats that resects ≈80% of the glandular portion leaving the forestomach almost intact (Glandular Gastrectomy,GG) and compared subsequent metabolic re-modeling with a sham-operation (SO).
GG did not affect body weight, at least after 10 weeks, improved hepatic and peripheral insulin sensitivity likely through the increased Akt, GSK-3 and AMPK phosphorylation, and reduced ectopic fat deposition and hepatic glycogen over-accumulation. The body adipose tissue was redistributed with reduction of intra-abdominal fat. We found reduction of circulating ghrelin levels, increased GLP1 plasma concentration and remodeling of the gut microbiome diversity, characterized by lower relative abundance of Ruminococcus and by higher relative abundance of Lactobacillus and Collinsella. These data suggest that, at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.
- Received January 9, 2016.
- Accepted July 8, 2016.
- © 2016 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.