TABLE 2

Effects of DNP on basal and stimulated insulin release

Glucose (mmol/l)DNP (μmol/l)Insulin release
n
pmol · g−1 · s−1osc/min
37.2 ± 0.80.22 ± 0.026
32509.6 ± 0.80.19 ± 0.033
11108 ± 25*0.19 ± 0.023
112508.8 ± 2.60.19 ± 0.043
  • Individual islets were perifused in the presence of 3 mmol/l glucose. DNP (250 μmol/l) was added to the perifusion medium either directly or after first elevating the glucose concentration to 11 mmol/l.

  • *

    * P < 0.001 vs. 3 mmol/l glucose alone;

  • P < 0.001 vs. 11 mmol/l glucose alone. osc, oscillations.