TABLE 3

Muscle glycogen, triglyceride, and total LCACoA content after hyperinsulinemic-euglycemic clamp

CH-ConHF-ConHF-AIC
White muscle
 Glycogen content (μmol/g)#49.6 ± 3.940.7 ± 2.1*53.7 ± 1.1
 Triglyceride content (μmol/g)#4.1 ± 1.85.6 ± 0.93.3 ± 1.0
 Total LCACoAs (nmol/g)#ND1.15 ± 0.131.19 ± 0.22
 Malonyl-CoA (nmol/g)0.9 ± 0.11.5 ± 0.11.1 ± 0.1§
Red muscle
 Glycogen content (μmol/g)#55.1 ± 1.851.3 ± 1.065.7 ± 4.1*
 Triglyceride content (μmol/g)#2.6 ± 0.23.4 ± 0.1*2.8 ± 0.5
 Total LCACoAs (nmol/g)#ND4.87 ± 0.614.57 ± 0.95
 Malonyl-CoA (nmol/g)1.1 ± 0.11.8 ± 0.11.0 ± 0.1
  • Data are means ± SE. Clamps were performed on standard diet-fed (CH-Con) or high-fat-fed rats 24h after AICAR (HF-AIC) or saline (HF-Con) injection. Total LCACoAs represent the sum of the three major species: linoleoyl-CoA, palmitoyl-CoA, and oleoyl-CoA. ND, not determined.

  • *

    * P < 0.05,

  • P < 0.01,

  • §

    § P < 0.05,

  • P < 0.01 vs HF-Con;

  • #

    # quadriceps;

  • gastrocnemius.