TABLE 2

Distribution of PKC isoforms in the membrane and cytosolic fractions

Glycerol


Lipid


0 h−2 h−6 h0 h−2 h−6 h
Membrane
 βI0.03 ± 0.030.05 ± 0.020.086 ± 0.050.05 ± 0.020.17 ± 0.030.52 ± 0.3
 ε0.27 ± 0.130.84 ± 0.750.16 ± 0.060.24 ± 0.110.55 ± 0.430.23 ± 0.05
 θ0.44 ± 0.180.98 ± 0.860.14 ± 0.040.23 ± 0.110.45 ± 0.290.20 ± 0.05
 ζ0.06 ± 0.010.07 ± 0.010.07 ± 0.010.10 ± 0.0010.10 ± 0.010.11 ± 0.01
Cytosol
 βI0.02 ± 0.020.04 ± 0.020.03 ± 0.010.06 ± 0.020.08 ± 0.020.07 ± 0.04
 ε0.34 ± 0.190.21 ± 0.080.22 ± 0.090.25 ± 0.110.18 ± 0.080.02 ± 0.01
 θ0.29 ± 0.110.14 ± 0.050.14 ± 0.060.15 ± 0.060.18 ± 0.060.13 ± 0.01
 ζ0.03 ± 0.010.05 ± 0.010.05 ± 0.010.06 ± 0.0030.04 ± 0.0030.05 ± 0.001
  • Data are means ± SE. n = 6 for PKC-θ and ε, and n = 3 of densitometric units for PKC-βI and -ε. See legend to Figs. 2C and D for details