TABLE 5

Effect of insulin infusion on Akt phosphorylation and protein expression

BasalTime (min)
3045–60
Phospho-Akt/protein (arbitrary units)
 Lean0.12 ± 0.050.83 ± 0.180.70 ± 0.12
 Obese0.06 ± 0.030.71 ± 0.080.40 ± 0.10*
Akt protein (arbitrary density units)
 Lean3.88 ± 0.683.00 ± 0.724.22 ± 0.68
 Obese4.46 ± 0.745.34 ± 1.04.38 ± 0.57
  • Data are means ± SE. For phospho-Akt/protein, data are presented as the ratio of the density of the anti-phospho-Ser473 blot to that of the anti-Akt protein expression blot. Akt protein is presented as arbitrary density units.

  • *

    * P < 0.05 vs. lean group;

  • P < 0.05 vs. 30-min time point for the obese group.