TABLE 2

Freshly dissociated and 12- to 96-h cultured VMH neurons assessed for expression of hexokinase I and glucokinase mRNA relative to cyclophilin

Timing/ treatmentChange in VMH gene expression
Hexokinase I/cyclophilin% freshGlucokinase/ cyclophilin% fresh
Freshly isolated0.88 ± 0.061.76 ± 0.2
12 h0.86 ± 0.04981.00 ± 0.09*57
24 h1.08 ± 0.041231.16 ± 0.06*66
48 h1.14 ± 0.051300.93 ± 0.11*53
72 h0.92 ± 0.051050.84 ± 0.08*48
96 h0.80 ± 0.04910.69 ± 0.11*39
  • Data are the means ± SE. n = 8 cultures/time period.

  • *

    * P ≤ 0.05 when values for neurons in culture were compared with those from freshly isolated neurons.