TABLE 3

Neurons transfected with glucokinase or nonsense siRNA after 24 h in culture and assessed for glucosensing

Timing/treatmentChange in glucosensing ability
NGlucose excited% freshGlucose inhibited% freshNonglucosensing
Freshly isolated75913 (97)16 (122)71 (540)
24 h cultures8315 (45)3810 (87)6384 (699)
48 h cultures3565 (18)388 (30)5087 (308)
96 h cultures1145 (5)389 (10)5687 (99)
24 h glucokinase siRNA2046 (12)4610 (20)6384 (172)
48 h glucokinase siRNA1584 (6)3113 (20)8184 (132)
96 h glucokinase siRNA660*01.5 (1)*998 (65)
96 h nonsense siRNA1037 (7)547 (7)4486 (89)
  • Data are N, % (n), and %. Neurons were assessed for glucosensing with Ca2+ imaging after 24, 48, and 96 h in culture. N, total number of neurons assessed at each time period; %, percent of neurons; n, number of neurons classified as glucose excited, glucose inhibited, and nonglucosensing at each time period.

  • *

    * P ≤ 0.05 when the percentage of neurons in the 96-h glucokinase siRNA group was compared with all other groups.

  • P ≤ 0.05 when the percentage of neurons in each glucosensing category was compared with that in freshly dissociated neurons by χ2 test.