TABLE 2

Insulin release from isolated pancreatic islets of type 2 diabetic patients and nondiabetic control subjects

Additions to the mediumNondiabeticType 2 diabetic
Glucose (3.3 mmol/l)6.3 ± 1.05.5 ± 0.3
Glucose (8.3 mmol/l)17.5 ± 1.3*4.7 ± 0.3
Glucose (16.7 mmol/l)24.3 ± 1.28.3 ± 1.6
Glucose (3.3 mmol/l) + GLP-1 (100 nmol/l)12.4 ± 2.36.5 ± 1.0
Glucose (16.7 mmol/l) + GLP-1 (100 nmol/l)32.7 ± 5.228.7 ± 4.1
Glucose (3.3 mmol/l) + glibenclamide (2 μmol/l)26.5 ± 5.14.7 ± 0.6
  • Data are means ± SE from diabetic patients (n = 4) and nondiabetic control subjects (n = 4). Insulin secretion (μU · islet−1 · h−1) was determined from three batches each of three isolated islets, incubated 60 min.

  • *

    * P < 0.01,

  • P < 0.001,

  • P < 0.05 vs. same type of islets at 3.3 mmol/l glucose.