TABLE 3

Gene expression of SNARE complex proteins in pancreatic islets from type 2 diabetic relative to nondiabetic patients

EnzymeMicroarray gene chip expressionQuantitative PCR
n43
Synaptotagmin V0.24 ± 0.02ND
Syntaxin-1A0.27 ± 0.024.0 × 10−2 (5.0 × 10−2–8.0 × 10−1)
SNAP-250.31 ± 0.079.5 × 10−3 (4.0 × 10−3–1.5 × 10−2)
nSec1 (munc-18)0.27 ± 0.057.3 × 10−2 (6.3 × 10−2–8.3 × 10−2)
Synaptophysin0.12 ± 0.021.5 × 10−2 (1.2 × 10−5–2.9 × 10−2)
VAMP-20.18 ± 0.03ND
PDX-10.97 ± 0.11ND
IRS20.70 ± 0.08ND
SLC2A2/GLUT20.17 ± 0.04ND
Glucokinase1.06 ± 0.07ND
KCNJ11/Kir6.20.61 ± 0.05ND
SUR10.58 ± 0.06ND
  • Data are means ± SE and means (range) and represent the ratio of diabetic to nondiabetic patients. ND, not determined.