TABLE 1

Cultured VMH neurons that were untreated or transfected with glucokinase or nonsense siRNA after 24 h in culture

Cell dishA (24 h postplating [baseline])B (24 h post-siRNA)B/A (%)C (72 h post-siRNA)C/A (%)D (72 h post-siRNA fura-2)D/C (%)
Culture time (h)24489696
Untreated (n)565089721296996
Nonsense siRNA (n)98707510811710093
Glucokinase siRNA (n)6063102941568793
  • Neurons were counted after 24, 48, and 96 h under phase contrast and at 96 h with fluorescence microscopy after loading cells with fura-2 at 96-h culture.