TABLE 1

Weight, wheel running, serum leptin, and adiposity in standard diet–and high-fat–raised rats

rAAV-controlrAAV-leptin
SedentaryWheel runningSedentaryWheel running
Standard diet–raised rats
    Body mass day 0 (g)483 ± 5484 ± 4486 ± 7488 ± 6
    Body mass day 51 (g)617 ± 10566 ± 13552 ± 16487 ± 23
    Wheel running (m/day)4167 ± 10914267 ± 1377
    Leptin day 0 (ng/ml)3.44 ± 0.533.94 ± 0.883.30 ± 0.463.14 ± 0.33
    Leptin day 51 (ng/ml)8.47 ± 1.364.34 ± 1.37*4.39 ± 1.250.71 ± 0.21*
    Adiposity (g)22.3 ± 1.413.6 ± 2.5§13.0 ± 2.4&2.4 ± 0.74§
High-fat–raised rats
    Body mass day 0 (g)702 ± 13701 ± 35708 ± 23695 ± 32
    Body mass day 51 (g)743 ± 14738 ± 22800 ± 32697 ± 27
    Wheel running (m/day)865 ± 265629 ± 212
    Leptin day 0 (ng/ml)24.4 ± 3.027.9 ± 4.528.4 ± 2.126.0 ± 2.9
    Leptin day 51 (ng/ml)26.1 ± 2.325.8 ± 4.441.4 ± 3.925.8 ± 4.1
    Adiposity (g)53.6 ± 5.250.6 ± 3.073.1 ± 6.947.2 ± 7.1
  • Data are means ± SE of six to eight rats per group. Adiposity represents the sum of PWAT, EWAT, and RTWAT. In three of eight standard diet–raised rats, we were unable to recover any PWAT or RTWAT; thus, in these rats, the adiposity is represented by EWAT only. Leptin day 51: standard diet raised: P = 0.003 for difference with leptin or wheel running by two-way ANOVA.

  • * P < 0.05 for difference from corresponding sedentary group by post-hoc analysis.

  • P < 0.05 for difference with leptin among sedentary groups by post-hoc analysis. High-fat raised: P < 0.05 for difference with leptin by two-way ANOVA.

  • P < 0.05 for difference from all other groups by post-hoc analysis. Adiposity: Standard diet raised: P = 0.001 for difference with leptin or wheel running by two-way ANOVA.

  • § P < 0.01 for difference from corresponding sedentary group by post-hoc analysis.

  • & P < 0.01 for difference with leptin among sedentary groups by post hoc analysis. High-fat raised: P < 0.05 for difference with wheel running by two-way ANOVA.

  • P < 0.05 for difference from corresponding sedentary group by post hoc analysis.