TABLE 1

Higher mitochondrial biogenesis in WAT of animals exposed to a soy-rich diet

Epididymal WAT
Muscle
Liver
Low phytoestrogenHigh phytoestrogenLow phytoestrogenHigh phytoestrogenLow phytoestrogenHigh phytoestrogen
Mitochondrial related regulation of transcription
    Tfam100 ± 28.5371.9 ± 36.3*100 ± 12.6144.1 ± 10.7100 ± 6.8105.7 ± 7.8
    ERRα100 ± 19.8132.2 ± 14.2100 ± 3.6134.4 ± 12.1100 ± 9.3110.0 ± 8.3
    PGC1b100 ± 23.0426.2 ± 72.9*100 ± 9.5155.6 ± 16.5100 ± 17.9174.1 ± 25.1*
ROS detoxifying enzymes
    Catalase100 ± 20.0219.3 ± 45.1100 ± 11.185.0 ± 8.7100 ± 7.4103.2 ± 7.1
    SOD1100 ± 19.8204.2 ± 25.8100 ± 6.2122.3 ± 4.8100 ± 7.1103.0 ± 6.8
    SOD2100 ± 18.0178.0 ± 21.7100 ± 7.3115.7 ± 9.2100 ± 4.391.8 ± 6.3
    Gpx1100 ± 26.7180.2 ± 28.6100 ± 9.0101.1 ± 6.6100 ± 9.088.9 ± 7.2
Mitochondrial respiratory chain
    ANT100 ± 18.9329.3 ± 33.6*100 ± 7.3115.6 ± 7.2100 ± 6.2116.7 ± 12.4
    ATP 5j100 ± 18.7316.8 ± 33.4*100 ± 9.9126.1 ± 8.8100 ± 8.7107.1 ± 7.6
    ATP synb100 ± 20.6187.0 ± 23.9100 ± 4.1100.8 ± 8.1100 ± 5.2118.5 ± 7.4
    COX II100 ± 21.6377.0 ± 36.8*100 ± 16.8166.9 ± 18.0100 ± 7.0122.0 ± 8.0
    COX III100 ± 30.0254.5 ± 27.8100 ± 9.0124.6 ± 11.2100 ± 8.1110.4 ± 8.1
    COX IV100 ± 20.8135.9 ± 15.5100 ± 4.8114.2 ± 10.3100 ± 5.7107.8 ± 7.3
    COX IV1100 ± 22.2124.7 ± 14.4100 ± 4.5124.5 ± 10.8100 ± 8.5117.1 ± 8.7
    COX 5b100 ± 19.3189.2 ± 22.5100 ± 7.1116.5 ± 9.2100 ± 6.7109.4 ± 6.6
    COX 6a100 ± 20.5202.2 ± 23.9100 ± 5.4105.6 ± 6.5100 ± 6.0105.6 ± 6.5
    COX 7a1100 ± 24.090.5 ± 10.2100 ± 6.4111.7 ± 10.6100 ± 12.0118.6 ± 13.5
    Cytc100 ± 17.2246.4 ± 30.5*100 ± 10.5110.3 ± 7.6100 ± 4.9105.3 ± 6.8
    Ndnfb5100 ± 21.7120.2 ± 16.3100 ± 5.6123.5 ± 14.0100 ± 7.884.6 ± 6.5
Uncoupling
    UCP2100 ± 10.789.9 ± 11.0100 ± 9.773.6 ± 9.2ndnd
    UCP3100 ± 21.5163.5 ± 21.7100 ± 13.6107.3 ± 21.60ndnd
Peroxisomal fatty acid oxidation
    ACoAx100 ± 17.7239.6 ± 26.3*100 ± 9.975.3 ± 11.6100 ± 18.8100.4 ± 14.2
  • Data are means ± SE, are summarized per group (n = 8–10), and are expressed as percentage relative to low-phytoestrogen tissues (100%). Quantitative real-time PCR of low- and high-phytoestrogen WAT, muscle, and liver. Normalization was performed using four housekeeping genes (Cyclophilin B, Actin-β, Tubulin-α, and Tubulin-β). t test,

  • *

    * P < 0.001,

  • P < 0.05,

  • P < 0.01 vs. control; nd, not determined.