TABLE 3

Fold difference in mRNA levels of genes regulating inflammation, metabolism, tissue remodeling, and antigen presentation in eATMΦ subtypes after 12 weeks of HFD relative to benchmark M2-polarized MGL1+/CD11c eATMΦs from mice fed an LFD for 8 weeks

8 weeks LFD12 weeks HFD
MGL1+ CD11cMGL1+ CD11cMGL1med CD11c+MGL1 CD11c+
Pro-/anti- Inflammatory markers
    Gene
        IL-13*8.15 ± 3.09†2.08 ± 0.66*0.15 ± 0.11‡
        Ym-1*7.98 ± 2.16†1.04 ± 0.18*0.68 ± 0.17*
        STAT6*5.24 ± 1.78†1.51 ± 0.25*0.86 ± 0.20*
        SPHK1*0.52 ± 0.08†0.41 ± 0.05†0.22 ± 0.02‡
        IL-10*1.32 ± 0.11†0.62 ± 0.12‡0.75 ± 0.20*‡
        IL-1Ra*2.94 ± 0.35†8.47 ± 1.56‡7.40 ± 1.01‡
        CD163*0.28 ± 0.01†0.13 ± 0.006‡0.02 ± 0.005‡
        CD206*0.57 ± 0.12†0.38 ± 0.03†0.22 ± 0.02‡
        Clec-7a*1.05 ± 0.18*1.36 ± 0.20*†1.84 ± 0.19†
        IL-1β*1.15 ± 0.12*0.95 ± 0.17*1.99 ± 0.21†
        Caspase-1*0.57 ± 0.04*0.54 ± 0.05*0.52 ± 0.05*
        IL-12p40*4.85 ± 1.24†6.80 ± 1.61†11.4 ± 3.53†
        TLR-4*0.91 ± 0.09*0.89 ± 0.09*0.56 ± 0.05†
        MCP-1*0.37 ± 0.10†0.21 ± 0.02†0.03 ± 0.004‡
Metabolism
    Genes
        ARG-1*0.70 ± 0.13*0.93 ± 0.17*0.98 ± 0.12*
        iNOS*0.53 ± 0.25*0.09 ± 0.01†0.03 ± 0.01‡
        PGC-1α*43.7 ± 13.6†283.4 ± 88.5‡107.8 ± 24.2‡
        PGC-1β*0.56 ± 0.07†0.56 ± 0.13†0.53 ± 0.14†
        ADRP*1.75 ± 0.14†2.26 ± 0.30†2.24 ± 0.26†
Remodeling
    Genes
        MMP-2*4.95 ± 0.91†6.39 ± 1.90†1.66 ± 0.48*
        MMP-12*40.7 ± 10.3†360 ± 55‡292 ± 46‡
        TGFβ-1*2.43 ± 0.35†2.36 ± 0.49†2.41 ± 0.42†
        VEGF*2.48 ± 0.17†5.48 ± 1.17‡8.95 ± 2.53‡
        ADAM-8*6.18 ± 0.38†14.28 ± 0.82‡14.57 ± 0.77‡
        LYVE-1*0.42 ± 0.15†0.26 ± 0.08†0.005 ± 0.001‡
Antigen presentation
    Genes
        I-a*‡0.71 ± 0.08*0.46 ± 0.08†0.99 ± 0.07‡
        CD80*0.37 ± 0.04†0.26 ± 0.04‡0.18 ± 0.04‡
        CD86*0.48 ± 0.05†0.17 ± 0.03‡0.10 ± 0.02‡
  • Data are means ± SE. Means designated by different symbols are significantly different (P < 0.05, ANOVA and Tukey procedure).