TABLE 1

Quantitative stereological analyses of the endocrine pancreas of GIPRdn transgenic pigs (tg) and wild-type control pigs (wt)

Parameter11 weeks (n = 5 wt, 5 tg)5 months (n = 4 wt, 4 tg)1–1.4 years (n = 5 wt, 5 tg)ANOVA
Means ± SEMeans ± SEMeans ± SEGroupAgeGroup × age
Pancreas weight (g)
    Wild type34.5 ± 4.2115.3 ± 5.6183.4 ± 13.8NS<0.0001NS
    Transgenic32.7 ± 3.1125.7 ± 6.1206.1 ± 4.9
VV(β-cell/islet) (%)
    Wild type69.8 ± 2.289.0 ± 1.590.2 ± 1.20.0066<0.00010.0024
    Transgenic70.8 ± 1.387.0 ± 1.276.4 ± 3.2*
VV(α-cell/islet) (%)
    Wild type14.1 ± 1.25.0 ± 0.85.0 ± 0.70.0122<0.00010.0008
    Transgenic12.2 ± 0.76.5 ± 0.713.8 ± 2.3*
VV(δ-cell/islet) (%)
    Wild type13.5 ± 2.84.3 ± 0.82.2 ± 0.6NS<0.0001NS
    Transgenic13.0 ± 1.05.5 ± 0.95.8 ± 0.9
VV(pp-cell/islet) (%)
    Wild type2.7 ± 0.71.8 ± 0.32.8 ± 0.8NS0.0355NS
    Transgenic3.9 ± 0.51.3 ± 0.33.8 ± 1.1
V(β-cell/islet) (mm3)
    Wild type168.7 ± 29.51,088.2 ± 82.01,694.6 ± 251.70.0002<0.00010.0024
    Transgenic152.1 ± 17.1664.1 ± 74.5663.7 ± 130.5*
V(α-cell/islet) (mm3)
    Wild type32.6 ± 8.758.4 ± 6.395.7 ± 17.4NS<0.0001NS
    Transgenic24.5 ± 1.747.7 ± 4.5112.8 ± 14.5
V(δ-cell/islet) (mm3)
    Wild type26.6 ± 3.949.5 ± 6.136.8 ± 6.3NS0.0014NS
    Transgenic25.9 ± 1.839.6 ± 3.547.5 ± 5.2
V(pp-cell/islet) (mm3)
    Wild type6.0 ± 1.820.7 ± 2.952.4 ± 12.6NS<0.0001NS
    Transgenic8.2 ± 1.79.3 ± 2.130.3 ± 7.9
  • Data were analyzed by the general linear models procedure (SAS Institute) taking the effects of group (wild type, transgenic), age (11 weeks, 5 months, 1–1.4 years), and the interaction group × age into account. For significant effects of these factors P values are indicated in the last three columns. In addition, significant differences between groups within age classes are marked by:

  • *P < 0.01;

  • P < 0.05;

  • NS, not significant.