TABLE 2

Biological parameters of HFLC and ob/ob and ob/+ mice

Number of miceControlHFLC
Weight (g)1232.3 ± 0.533.0 ± 0.6
Weight gain (g)124.7 ± 0.44.6 ± 0.3
GIR (mg ⋅ kg–1 ⋅ min−1)531 ± 73.5 ± 3.5*
Fasting plasma insulin (mU/L)54.1 ± 0.911.0 ± 0.5*
Fasting blood glucose (mmol/L)56.0 ± 0.38.0 ± 0.7*
HOMA-IR51.1 ± 0.13.9 ± 0.1*
ob/+ob/ob
Weight (g)824.1 ± 0.445.4 ± 1.0
Fasting plasma insulin (mU/L)53.5 ± 0.1104.0 ± 14.5
Fasting blood glucose (mmol/L)56.9 ± 0.69.1 ± 0.3
AUC OGTT83.9 ± 0.26.4 ± 0.4
OGTT insulin (mU/L; 30 min)529.6 ± 4.3136.4 ± 3.2
HOMA-IR51.1 ± 0.141.8 ± 6.5
HOMA-IRmet50.7 ± 0.227.0 ± 5.8
  • Data are means ± SEM. Bl/6 mice were fed either the M25 control diet or an HFLC diet. Insulin was measured by enzyme-linked immunosorbent assay. HOMA-IR was calculated using the formula [glucose (mmol/L) × insulin (mU/L)/22.5]. HOMA-IRmet was measured in mice treated for 10 days with 25 mg/kg metformin twice a day. AUC OGTT, area under curve of blood glucose concentrations after 4 mg/kg oral glucose bolus. Plasma insulin (mU/L) was at 30 min during OGTT. GIR, glucose infusion rate in condition of a hyperinsulinemic-euglycemic clamp after 5 months.

  • *P < 0.05.

  • P < 0.01.