Table 1

Peptides identified from a 48-h digest of mouse aortic elastin with NE

ResidueMice aortaMr (Da)
StartEnd
3746GLPGGVPGGV808.44
8093FGAGPGGLGGAGPG1,070.51
8094FGAGPGGLGGAGPGA1,141.55
8096FGAGPGGLGGAGPGAGL1,311.66
8097FGAGPGGLGGAGPGAGLG1,368.68
8194GAGPGGLGGAGPGA994.48
8196GAGPGGLGGAGPGAGL1,164.59
136144GGVPGGVGV697.38
181191STGAVVPQVGA984.52
205213VGLPGVYPG873.46
210218VYPGGVLPG857.46
251264GIPGVGPFGGQQPG1,282.63
251267GIPGVGPFGGQQPGVPL1,591.83
325342GGAGVLPGVGGGGIPGGA1,348.71
328342GVLPGVGGGGIPGGA1,179.62
328342GVLPGVGGGGIPGGA1,163.63
453462GAGGFPGYGV880.41
455462GGFPGYGV752.35
493503LGGLVPGAVPG935.54
512525VPGAGGVPGAGTPA1,106.57
540552GLGPGVGGVPGGV1,021.56
542552GPGVGGVPGGV851.45
558575PGGVGVGGVPGGVGPGGV1,390.72
558575PGGVGVGGVPGGVGPGGV1,390.72
564575GGVPGGVGPGGV908.47
564575GGVPGGVGPGGV924.47
576588TGIGAGPGGLGGA983.50
576592TGIGAGPGGLGGAGSPA1,295.65
576592TGIGAGPGGLGGAGSPA1,311.64
577588GIGAGPGGLGGA882.46
577592GIGAGPGGLGGAGSPA1,194.60
579592GAGPGGLGGAGSPA1,024.49
611621GLGAGVPGFGA901.47
611621GLGAGVPGFGA917.46
  • Bioactive motifs are highlighted in bold. Hydroxylated proline residues are shown in italic/underline. Digests of the mouse aortic samples were analyzed by nanoHPLC-nanoESI-QqTOF-MS/MS. In brief, chromatographic separation was performed using a binary reverse-phase gradient, and peptides in the mass-to-charge ratio range 300–2,500 were selected for MS/MS in data-directed acquisition mode. All fragment spectra were preprocessed using proprietary software, and the resultant peak lists were subjected to automated de novo sequencing using Peaks Studio 7.0 (Bioinformatics Solutions, Waterloo, ON, Canada) with subsequent database matching using a SwissProt database.