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Original contribution

Erythrocyte and Monocyte Insulin Binding in Man: A Comparative Analysis in Normal and Disease States

  1. Robert F Dons,
  2. Janice Ryan,
  3. Phillip Gorden and
  4. Helena Wachslicht-Rodbard
  1. Diabetes Branch, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, National Institutes of Health Bethesda, Maryland
  1. Address reprint requests to Dr. Phillip Gordon, Diabetes Branch, NIADDK, Building 10, Room8S-243, National Institutes of Health, Bethesda, Maryland 20205.
Diabetes 1981 Nov; 30(11): 896-902. https://doi.org/10.2337/diab.30.11.896
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Abstract

Blood cells have been widely used to evaluate the status of the insulin receptor in man. The insulin receptor of human monocytes has been shown to mirror pathophysiologic states of insulin receptors in target tissues of animal models. Thus, comparison of the properties of the insulin receptor studied simultaneously in erythrocytes and monocytes is appropriate. We studied 19 normal subjects and 32 patients with diverse conditions such as acromegaly, insulinomas, insulin-dependent diabetes mellitus, corticosteroid administration, and the Type A and B forms of insulin resistance. We find that specific insulin binding at tracer concentrations significantly correlate (P < 0.001) for the two cell types obtained from these individuals. However, the total number of binding sites (Ro) and affinity of the receptor at the lowest insulin concentration (Ke) do not significantly correlate (P < 0.1). Monocytes from patients with the Type A and B forms of insulin resistance exhibit marked alterations in insulin binding and simultaneous studies in erythrocytes reflect these changes. The administration of 40 mg/day of prednisone for 3 days to normal subjects produced no significant change in insulin binding to either cell type. Insulin binding to both cell types from poorly controlled insulin-dependent diabetes mellitus patients was similarly normal or elevated. However, improved control decreased insulin binding in monocytes, but not in erythrocytes. We conclude that there is a general relationship between monocyte and erythrocyte insulin binding, but important differences exist in the way their insulin receptors are regulated. Insulin binding in erythrocytes is inversely and exponentially related to cell age. Unlike erythrocytes, monocytes represent a more uniform population of cells capable of the same receptor-mediated endocytotic functions as hepatocytes. Thus, the monocytemay provide a clearer reflection of the insulin receptor status in target tissues.

  • Received March 13, 1981.
  • Copyright © 1981 by the American Diabetes Association

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November 1981, 30(11)
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Erythrocyte and Monocyte Insulin Binding in Man: A Comparative Analysis in Normal and Disease States
Robert F Dons, Janice Ryan, Phillip Gorden, Helena Wachslicht-Rodbard
Diabetes Nov 1981, 30 (11) 896-902; DOI: 10.2337/diab.30.11.896

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Erythrocyte and Monocyte Insulin Binding in Man: A Comparative Analysis in Normal and Disease States
Robert F Dons, Janice Ryan, Phillip Gorden, Helena Wachslicht-Rodbard
Diabetes Nov 1981, 30 (11) 896-902; DOI: 10.2337/diab.30.11.896
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