Skip to main content
  • More from ADA
    • Diabetes Care
    • Clinical Diabetes
    • Diabetes Spectrum
    • ADA Standards of Medical Care in Diabetes
    • ADA Scientific Sessions Abstracts
    • BMJ Open Diabetes Research & Care
  • Subscribe
  • Log in
  • My Cart
  • Follow ada on Twitter
  • RSS
  • Visit ada on Facebook
Diabetes

Advanced Search

Main menu

  • Home
  • Current
    • Current Issue
    • Online Ahead of Print
    • ADA Scientific Sessions Abstracts
  • Browse
    • By Topic
    • Issue Archive
    • Saved Searches
    • ADA Scientific Sessions Abstracts
  • Info
    • About the Journal
    • About the Editors
    • ADA Journal Policies
    • Instructions for Authors
    • Guidance for Reviewers
  • Reprints/Reuse
  • Advertising
  • Subscriptions
    • Individual Subscriptions
    • Institutional Subscriptions and Site Licenses
    • Access Institutional Usage Reports
    • Purchase Single Issues
  • Alerts
    • E­mail Alerts
    • RSS Feeds
  • Podcast
  • Submit
    • Submit a Manuscript
    • Submit Cover Art
    • ADA Journal Policies
    • Instructions for Authors
    • ADA Peer Review
  • More from ADA
    • Diabetes Care
    • Clinical Diabetes
    • Diabetes Spectrum
    • ADA Standards of Medical Care in Diabetes
    • ADA Scientific Sessions Abstracts
    • BMJ Open Diabetes Research & Care

User menu

  • Subscribe
  • Log in
  • My Cart

Search

  • Advanced search
Diabetes
Advertisement
  • Home
  • Current
    • Current Issue
    • Online Ahead of Print
    • ADA Scientific Sessions Abstracts
  • Browse
    • By Topic
    • Issue Archive
    • Saved Searches
    • ADA Scientific Sessions Abstracts
  • Info
    • About the Journal
    • About the Editors
    • ADA Journal Policies
    • Instructions for Authors
    • Guidance for Reviewers
  • Reprints/Reuse
  • Advertising
  • Subscriptions
    • Individual Subscriptions
    • Institutional Subscriptions and Site Licenses
    • Access Institutional Usage Reports
    • Purchase Single Issues
  • Alerts
    • E­mail Alerts
    • RSS Feeds
  • Podcast
  • Submit
    • Submit a Manuscript
    • Submit Cover Art
    • ADA Journal Policies
    • Instructions for Authors
    • ADA Peer Review
Original contribution

Effects of Experimental Diabetes and Insulin on Cyclic AMP Phosphodiesterase and its Protein Activator in Rat Adipose Tissue

  1. Solomon S Solomon,
  2. Marjorie Palazzolo,
  3. Janet McPherson and
  4. Al Smoake
  1. Research Service, Department of Medicine, VA Medical Center and University of Tennessee Center for the Health Sciences Memphis, Tennessee
  2. New Mexico Institute of Mining and Technology Socorro, New Mexico
  1. Address reprint requests to Solomon S. Solomon, 1030 Jefferson Avenue, Memphis, Tennessee 38104.
Diabetes 1981 May; 30(5): 372-376. https://doi.org/10.2337/diab.30.5.372
PreviousNext
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Streptozotocin-induced diabetes in the rat has been shown to decrease and insulin to increase the activity of cyclic AMP phosphodiesterase (PDE) in adipose tissue. Protein activator (PA), a substance of low molecular weight, is essential for full activity of some component phosphodiesterases. A significant decrease in protein activator level (60% of control) is found in the 13,000 × g boiled supernatant from streptozotocin-diabetic rat adipose tissue. When diabetic animals are treated with insulin 24 h following the induction of diabetes, protein activator is restored to 80% of the normal control level; when diabetic animals are immediately treated with insulin, the levels of protein activator are not reduced (98% of control). Hence, insulin is capable of reversing both the decrease in protein activator and phosphodiesterase activity seen with diabetes. When partially purified supernatant from diabetic animal fat is chromatographed on a DEAE Sephacel column, the untreated diabetics show inhibition, whereas insulin-treated diabetics show maximal stimulation of the low Km cyclic AMP phosphodiesterase. Protein activator and an inhibitor are readily separated when adipose tissue homogenate is centrifuged at 13,000 × g and the boiled supernatant is passed over a DEAE Sephacel column.

Diabetes is associated with a decrease in both cyclic AMP phosphodiesterase and protein activator activity, both of which are restored to normal by administration of insulin. We also report the presence of an inhibitor of activator, which is increased in streptozotocin diabetes. All of these components appear to play a role in the pathophysiology of diabetes.

  • Received January 21, 1980.
  • Revision received December 1, 1980.
  • Accepted December 1, 1980.
  • Copyright © 1981 by the American Diabetes Association

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top
Advertisement

In this Issue

May 1981, 30(5)
  • Table of Contents
  • Index by Author
Sign up to receive current issue alerts
View Selected Citations (0)
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about Diabetes.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Effects of Experimental Diabetes and Insulin on Cyclic AMP Phosphodiesterase and its Protein Activator in Rat Adipose Tissue
(Your Name) has forwarded a page to you from Diabetes
(Your Name) thought you would like to see this page from the Diabetes web site.
Citation Tools
Effects of Experimental Diabetes and Insulin on Cyclic AMP Phosphodiesterase and its Protein Activator in Rat Adipose Tissue
Solomon S Solomon, Marjorie Palazzolo, Janet McPherson, Al Smoake
Diabetes May 1981, 30 (5) 372-376; DOI: 10.2337/diab.30.5.372

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Add to Selected Citations
Share

Effects of Experimental Diabetes and Insulin on Cyclic AMP Phosphodiesterase and its Protein Activator in Rat Adipose Tissue
Solomon S Solomon, Marjorie Palazzolo, Janet McPherson, Al Smoake
Diabetes May 1981, 30 (5) 372-376; DOI: 10.2337/diab.30.5.372
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Nutrition and Somatomedin: XVII. Circulating Somatomedin C During Treatment of Diabetic Ketoacidosis
  • Primary Hypoandrogenism in Experimental Diabetes in the Long-Evans Rat
  • Subunit Structure, Autophosphorylation, and Tyrosine-Specific Protein Kinase Activity of Hepatic Insulin Receptors in Fetal, Neonatal, and Adult Rats
Show more Original contribution

Similar Articles

Navigate

  • Current Issue
  • Online Ahead of Print
  • Scientific Sessions Abstracts
  • Collections
  • Archives
  • Submit
  • Subscribe
  • Email Alerts
  • RSS Feeds

More Information

  • About the Journal
  • Instructions for Authors
  • Journal Policies
  • Reprints and Permissions
  • For Advertisers
  • Privacy Policy: ADA Journals
  • Copyright Notice/Public Access Policy
  • Contact Us

Other ADA Resources

  • Diabetes Care
  • Clinical Diabetes
  • Diabetes Spectrum
  • Standards of Medical Care in Diabetes
  • BMJ Open - Diabetes Research & Care
  • Scientific Sessions Abstracts
  • Professional Books
  • Diabetes Forecast

 

  • DiabetesJournals.org
  • Diabetes Core Update
  • ADA's DiabetesPro
  • ADA Member Directory
  • Diabetes.org
Advertisement

© 2019 by the American Diabetes Association. Diabetes Print ISSN: 0012-1797, Online ISSN: 1939-327X.