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Original Contribution

Effect of Culture Conditions on Fetal Mouse Pancreas In Vitro and After Transplantation in Syngeneic and Allogeneic Recipients

  1. T E Mandel and
  2. M Koulmanda
  1. Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital Victoria 3050, Australia
  1. Address reprint requests to Dr. T. E. Mandel at the above address.
Diabetes 1985 Nov; 34(11): 1082-1087. https://doi.org/10.2337/diab.34.11.1082
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Abstract

Organ culture of fetal mouse pancreas under conditions designed to either maximally preserve islet cell survival or reduce immunogenicity was used to test the efficacy of islet graft function in diabetic syngeneic or fully allogeneic recipients. Organ culture in either 90% O2, or at low temperature (22°C), or in a combination of 90% O2 and 22°C for 14 days not only failed to reduce immunogenicity in fully allogeneic recipients but also diminished endocrine cell survival in grafts in syngeneic recipients. Monitoring of in vitro insulin secretion provided a better guide to future graft function than did the insulin content of the cultured tissue. It is concluded that culture conditions that have been shown to reduce immunogenicity (high O2 concentration and low temperature) are not synergistic when used together and are also potentially damaging to endocrine cells.

  • Received February 21, 1985.
  • Revision received April 29, 1985.
  • Copyright © 1985 by the American Diabetes Association

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November 1985, 34(11)
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Effect of Culture Conditions on Fetal Mouse Pancreas In Vitro and After Transplantation in Syngeneic and Allogeneic Recipients
T E Mandel, M Koulmanda
Diabetes Nov 1985, 34 (11) 1082-1087; DOI: 10.2337/diab.34.11.1082

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Effect of Culture Conditions on Fetal Mouse Pancreas In Vitro and After Transplantation in Syngeneic and Allogeneic Recipients
T E Mandel, M Koulmanda
Diabetes Nov 1985, 34 (11) 1082-1087; DOI: 10.2337/diab.34.11.1082
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