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Original Contribution

Insulin, via Its Own Receptor, Regulates Growth and Amylase Synthesis in Pancreatic Acinar AR42J Cells

  1. Joachim Mössner,
  2. Craig D Logsdon,
  3. John A Williams and
  4. Ira D Goldfine
  1. Cell Biology Research Laboratory Mount Zion Hospital and Medical Center P.O. Box 7921, San Francisco, California Departments of Physiology and Medicine, University of California San Francisco, California
  1. Address reprint requests to Dr. Joachim Mössner, University of Würzburg, Medical Poliklinik, Klinikstrasse 8, Würzburg 8700, FRG.
Diabetes 1985 Sep; 34(9): 891-897. https://doi.org/10.2337/diab.34.9.891
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Abstract

Previous in vivo studies have suggested a long-term regulatory role for insulin in the exocrine pancreas. To directly study the long-term effects of insulin on the pancreas in vitro, we have used cultured AR42J cells, a rat cell line that is derived from a transplantable tumor of the acinar pancreas. Hormone-binding experiments with 125I-labeled hormones indicated that AR42J cells have insulin receptors, relatively fewer receptors for insulin-like growth factor II (IGF-II), and no detectable receptors for insulin-like growth factor I (IGF-I). Insulin at concentrations as low as 1 nM stimulated the growth of these cells, as measured by an increase in DNA and protein content, and in cell number. At 100 nM, where insulin had a maximal effect, the growth of AR42J cells was stimulated by 46.1 ± 10.9% (mean ± SEM, N = 11).Insulin increased the amylase activity of AR42J cells over the same concentration range that it stimulated growth; at 100 nM, insulin increased amylase by 91.0 ± 15.4% (mean ± SEM, N = 23). Immunoprecipitation of [35S]methionine-labeled proteins revealed that insulin induced a selective increase of amylase synthesis over general protein synthesis. These studies indicate, therefore, that insulin stimulates both growth and amylase synthesis of AR42J cells.

  • Received July 28, 1984.
  • Revision received January 12, 1985.
  • Copyright © 1985 by the American Diabetes Association

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September 1985, 34(9)
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Insulin, via Its Own Receptor, Regulates Growth and Amylase Synthesis in Pancreatic Acinar AR42J Cells
Joachim Mössner, Craig D Logsdon, John A Williams, Ira D Goldfine
Diabetes Sep 1985, 34 (9) 891-897; DOI: 10.2337/diab.34.9.891

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Insulin, via Its Own Receptor, Regulates Growth and Amylase Synthesis in Pancreatic Acinar AR42J Cells
Joachim Mössner, Craig D Logsdon, John A Williams, Ira D Goldfine
Diabetes Sep 1985, 34 (9) 891-897; DOI: 10.2337/diab.34.9.891
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