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Original Articles

Age-Dependent Stimulation of Neonatal Insulin Release and Inositol Phosphate Accumulation by CCK-8 and Carbachol

  1. Donald J Fletcher,
  2. Wesley H Rowley,
  3. Susan J Pabst and
  4. Jack E Brinn
  1. Department of Anatomy and Cell Biology, School of Medicine, East Carolina University Greenville, North Carolina
  1. Address correspondence and reprint requests to Donald J. Fletcher, Department of Anatomy and Cell Biology, School of Medicine, East Carolina University, Greenville, NC 27858-4354.
Diabetes 1989 Nov; 38(11): 1337-1342. https://doi.org/10.2337/diab.38.11.1337
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Abstract

Development of a robust insulin secretory response to glucose occurs during the early neonatal period. To determine if neuroendocrine agents play a role during this time, we studied the effects of selected peptides and neurotransmitters on insulin release and polyphosphoinositide metabolism in islets isolated from 1- and 3-day neonatal rats. Vasoactive intestinal peptide had no effect on glucose-stimulated release in either islet population. In contrast, sulfated cholecystokinin octapeptide (CCK-8) significantly enhanced glucose-induced insulin release in both islet groups. One-day islets were stimulated only by a concentration of 300 nM, whereas 3-day islets were responsive at 3 nM. Similar to CCK-8, there were clear differences in responses to carbachol between 1- and 3-day islets. One-day islets required a concentration of 200 μM for insulin release to be significantly greater than with glucose alone; 3-day islet insulin release was significant at 2 μM carbachol. Both agonists stimulated inositol phosphate accumulation in 3-day islets, but only CCK-8 caused a significant increase over glucose-induced levels in 1-day islets. These results indicate that islet responsiveness to CCK-8 and carbachol develops in parallel during the early neonatal period. This development may be linked to the maturation of a critical step of stimulus-secretion coupling through which these agents act.

  • Received November 7, 1988.
  • Revision received June 26, 1989.
  • Accepted June 26, 1989.
  • Copyright © 1989 by the American Diabetes Association

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November 1989, 38(11)
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Age-Dependent Stimulation of Neonatal Insulin Release and Inositol Phosphate Accumulation by CCK-8 and Carbachol
Donald J Fletcher, Wesley H Rowley, Susan J Pabst, Jack E Brinn
Diabetes Nov 1989, 38 (11) 1337-1342; DOI: 10.2337/diab.38.11.1337

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Age-Dependent Stimulation of Neonatal Insulin Release and Inositol Phosphate Accumulation by CCK-8 and Carbachol
Donald J Fletcher, Wesley H Rowley, Susan J Pabst, Jack E Brinn
Diabetes Nov 1989, 38 (11) 1337-1342; DOI: 10.2337/diab.38.11.1337
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