Cloning and Expression of IDDM-Specific Human Autoantigens

Daniel U Rabin; Susan M Pleasic; Rebecca Palmer-Crocker; Jeffrey A Shapiro

doi:10.2337/diab.41.2.183

Original Articles

Cloning and Expression of IDDM-Specific Human Autoantigens

Daniel U Rabin,
Susan M Pleasic,
Rebecca Palmer-Crocker and
Jeffrey A Shapiro

Molecular Diagnostics, Inc. West Haven, Connecticut

Address correspondence and reprint requests to Daniel U. Rabin, Molecular Diagnostics, Inc., Miles Research Center, 400 Morgan Lane, West Haven, CT 06516.

Diabetes 1992 Feb; 41(2): 183-186. https://doi.org/10.2337/diab.41.2.183

Abstract

A DNA cloning approach was taken to identify islet cell protein antigens that are recognized specifically by insulin-dependent diabetes mellitus (IDDM) sera. A human islet cDNA library was generated and screened with diabetic sera. In this article, identification of two clones is described. Proteins expressed by these λ phages appeared to react specifically with newly diagnosed diabetic sera. Islet cell antibody 12 (ICA12) was tested by Western blotting. ICA512 was not reactive with sera in the Western format but was specifically immunoprecipitated by diabetic sera from an Escherichia coli extract.