Multiple TCR Vβ Usage by Infiltrates of Young NOD Mouse Islets of Langerhans: A Polymerase Chain Reaction Analysis
Abstract
Because a restricted repertoire of T-cell receptor (TCR) Vβp gene expression has been reported in other autoimmune diseases, the possibility of similarly restricted Vα gene expression by T-cell infiltrates of NOD mouse islets was examined. With isolated islets from 4- to 12-wk-old NOD mice, a prospective polymerase chain reaction analysis with 18 Vβ-specific oligonucleotide primers was performed on the noncloned and unexpanded islet-infiltrating T cells. The methodology used permitted the detection of a minimum of 50 T cells. In contrast to the restricted TCR Vβ gene usage reported for other autoimmune diseases, infiltrates of even the youngest mice were characterized by expression of multiple Vβ gene segments.
- Received September 23, 1991.
- Revision received November 22, 1991.
- Accepted November 22, 1991.
- Copyright © 1992 by the American Diabetes Association
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In this Issue
March 1992, 41(3)
Multiple TCR Vβ Usage by Infiltrates of Young NOD Mouse Islets of Langerhans: A Polymerase Chain Reaction Analysis
