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Original Articles

Glucose-6-Phosphatase mRNA and Activity Are Increased to the Same Extent in Kidney and Liver of Diabetic Rats

  1. Gilles Mithieux,
  2. Hubert Vidal,
  3. Carine Zitoun,
  4. Nathalie Bruni,
  5. Nathalie Daniele and
  6. Carol Minassian
  1. Institut National de la Santé et de la Recherche Médicale, Faculty of Medicine Alexis Carrel Lyon, France
  1. Address correspondence and reprint requests to Gilles Mithieux, Institut National de la Santé et de la Recherche Médicale, U449, Faculté de Médecine Alexis Carrel, rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
Diabetes 1996 Jul; 45(7): 891-896. https://doi.org/10.2337/diab.45.7.891
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Abstract

Using Northern blot with a specific glucose-6-phosphatase (Glc6Pase) cDNA probe and enzymatic activity determination, we studied the effect of streptozotocininduced diabetes on Glc6Pase in rat gluconeogenic tissues. The Glc6Pase mRNA abundance was increased four to five times in both the liver and kidney of diabetic rats. This was correlated with a concomitant 130% increase in Glc6Pase catalytic subunit in both tissues. The elevated level of Glc6Pase mRNA was significantly corrected in both the liver and kidney of diabetic rats after a 12-h insulin treatment. We also studied Glc6Pase mRNA and activity in gluconeogenic tissues during the fed-fasted and fasted-refed transitions in normal rats. In the liver, the abundance of Glc6Pase mRNA was sharply increased about four times after 24 or 48 h of fasting. In the kidney, the Glc6Pase mRNA level was gradually increased some three and five times after 24 and 48 h of fasting, respectively. The increase of Glc6Pase mRNA in both organs was matched with a doubling of the activity of Glc6Pase catalytic subunit: rapid in the liver and gradual in the kidney. The liver Glc6Pase mRNA abundance in 48-h fasted rats was acutely and importantly decreased upon refeeding. The kidney Glc6Pase mRNA level was also significantly lowered under these conditions, albeit less rapidly. These data demonstrate that efficient control of Glc6Pase takes place in both gluconeogenic organs at the pretranslational level and suggest that insulin might play an important role in this control. In addition, using reverse transcription-polymerase chain reaction and Northern blot, we report that Glc6Pase mRNA is not detectable in several other tissues previously assumed to express the enzyme.

  • Received November 21, 1995.
  • Revision received February 22, 1996.
  • Accepted February 22, 1996.
  • Copyright © 1996 by the American Diabetes Association

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July 1996, 45(7)
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Glucose-6-Phosphatase mRNA and Activity Are Increased to the Same Extent in Kidney and Liver of Diabetic Rats
Gilles Mithieux, Hubert Vidal, Carine Zitoun, Nathalie Bruni, Nathalie Daniele, Carol Minassian
Diabetes Jul 1996, 45 (7) 891-896; DOI: 10.2337/diab.45.7.891

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Glucose-6-Phosphatase mRNA and Activity Are Increased to the Same Extent in Kidney and Liver of Diabetic Rats
Gilles Mithieux, Hubert Vidal, Carine Zitoun, Nathalie Bruni, Nathalie Daniele, Carol Minassian
Diabetes Jul 1996, 45 (7) 891-896; DOI: 10.2337/diab.45.7.891
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