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Original Articles

Temporal and Quantitative Correlations Between Insulin Secretion and Stably Elevated or Oscillatory Cytoplasmic Ca2+ in Mouse Pancreatic β-Cells

  1. Jean-Christophe Jonas,
  2. Patrick Gilon and
  3. Jean-Claude Henquin
  1. Unité d'Endocrinologie et Métabolisme, University of Louvain Faculty of Medicine Brussels, Belgium
  1. Address correspondence and reprint requests to Dr. J.C. Henquin,Unite d'Endocrinologie et Metabolisme, UCL 55.30, Avenue Hippocrate 55, B-1200 Brussels, Belgium.
Diabetes 1998 Aug; 47(8): 1266-1273. https://doi.org/10.2337/diab.47.8.1266
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Abstract

An increase in cytoplasmic Ca2+ in β-cells is a key step in glucose-induced insulin secretion. However, whether changes in cytoplasmic free Ca2+ ([Ca2+]i) directly regulate secretion remains disputed. This question was addressed by investigating the temporal and quantitative relationships between [Ca2+]i and insulin secretion. Both events were measured simultaneously in single mouse islets loaded with fura-PE3 and perifused with a medium containing diazoxide (to prevent any effect of glucose on the membrane potential) and either 4.8 or 30 mmol/l K+. Continuous depolarization with 30 mmol/l K+ in the presence of 15 mmol/l glucose induced a sustained rise in [Ca2+]i and insulin release. No oscillations of secretion were detected even after mathematical analysis of the data (pulse, spectral and sample distribution analysis). In contrast, alternating between 30 and 4.8 mmol/l K+ (1 min/2 min or 2.5 min/5 min) triggered synchronous [Ca2+]i and insulin oscillations of regular amplitude in each islet. A good correlation was found between [Ca2+]i and insulin secretion, and it was independent of the presence or absence of oscillations. This quantitative correlation between [Ca2+]i and insulin secretion was confirmed by experiments in which extracellular Ca2+ was increased or decreased (0.1–2.5 mmol/l) stepwise in the presence of 30 mmol/l K+. This resulted in parallel stepwise increases or decreases in [Ca2+]i and insulin secretion. However, while the successive [Ca2+]i levels were unaffected by glucose, each plateau of secretion was much higher in 20 than in 3 mmol/l glucose. In conclusion, in our preparation of normal mouse islets, insulin secretion oscillates only when [Ca2+]i oscillates in β-cells. This close temporal relationship between insulin secretion and [Ca2+]i changes attests of the regulatory role of Ca2+. There also exists a quantitative relationship that is markedly influenced by the concentration of glucose.

  • Received November 14, 1997.
  • Revision received May 6, 1998.
  • Accepted May 6, 1998.
  • Copyright © 1998 by the American Diabetes Association

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August 1998, 47(8)
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Temporal and Quantitative Correlations Between Insulin Secretion and Stably Elevated or Oscillatory Cytoplasmic Ca2+ in Mouse Pancreatic β-Cells
Jean-Christophe Jonas, Patrick Gilon, Jean-Claude Henquin
Diabetes Aug 1998, 47 (8) 1266-1273; DOI: 10.2337/diab.47.8.1266

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Temporal and Quantitative Correlations Between Insulin Secretion and Stably Elevated or Oscillatory Cytoplasmic Ca2+ in Mouse Pancreatic β-Cells
Jean-Christophe Jonas, Patrick Gilon, Jean-Claude Henquin
Diabetes Aug 1998, 47 (8) 1266-1273; DOI: 10.2337/diab.47.8.1266
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