Vascular NADH Oxidase Is Involved in Impaired Endothelium-Dependent Vasodilation in OLETF Rats, a Model of Type 2 Diabetes
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Figures
- FIG. 1.
Vascular relaxations after treatment with ACh (A) or SNP (B) in the precontracted aortic rings from LETO and OLETF rats were recorded in the presence (•, ▪) or absence (○, □) of SOD (500 units/ml). Data are means ± SE of eight experiments. *P < 0.05, **P < 0.01 compared with LETO (Vehicle) rats. #P < 0.05 compared with corresponding values of OLETF (Vehicle) rats.
- FIG. 2.
Comparison of densities of immunohistochemical stain for eNOS in aortas from LETO and OLETF rats. The density of eNOS staining in aortic cross sections was expressed as pixels per micrometers squared of tissue.
- FIG. 3.
Vascular superoxide production in aortic segments from LETO and OLETF rats was assessed by lucigenin chemiluminescence assay in the absence (Vehicle) or presence of DPI (10 μmol/l). Data are expressed as nanomoles per minute per milligram dry weight of vessels. Bars are means ± SE of four experiments in each group. **P < 0.01 compared with LETO rats; ##P < 0.01 compared with vehicle of OLETF rats.
- FIG. 4.
Production of superoxide anion in vascular homogenates (A), and cytosolic (B) and particulate (C) fractions in response to several potential substrates, NADH (100 μmol/l), NADPH (100 μmol/l), arachidonic acid (AA; 100 μmol/l), xanthine (100 nmol/l), and succinate (5 mmol/l). Bars are means ± SE of four experiments in each group. *P < 0.05, **P < 0.01 compared with LETO rats.
- FIG. 5.
Effects of various inhibitors, such as DPI (10 μmol/l), SOD (500 units/ml), allopurinol (100 μmol/l), rotenone (100 μmol/l), l-NMMA (10 μmol/l), and indomethacin (10 μmol/l), on NADH-stimulated superoxide production in particulate fractions. Bars are means ± SE of four experiments in each group. *P < 0.05, **P < 0.01, #P < 0.05 compared with corresponding values in vehicle.
- FIG. 6.
The expression of p22phox mRNA in the thoracic aorta derived from LETO and OLETF rats. The top panel shows a representative Northern blot with the p22phox mRNA band at 0.8 kb. Ethidium bromide staining of the 28S and 18S ribosomal RNA is shown in the lower panel. This was used to normalize small differences in loading of total RNA or the efficiency of mRNA transfer to the membrane in each lane. **P < 0.01 compared with LETO rats.
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