Morphometry of Dorsal Root Ganglion in Chronic Experimental Diabetic Neuropathy
Article Figures & Tables
Figures
- FIG. 1.
Dorsal root ganglion neurons of control (A) and diabetic (B) rats. Diabetic neurons tend to be small and more basophilic and have more and larger vacuoles and pigmentary inclusions. Bar represents 25 μm.
- FIG. 2.
Composite histogram of L5 dorsal root ganglia of control and diabetic rats.
- FIG. 3.
Diameter histograms of control and diabetic ganglia for all neurons (A) and two groups of large neurons (B). *P < 0.05; **P < 0.01.
Tables
- TABLE 1
Electrophysiology of 12-month diabetic and age-matched control rats
Group n Digital nerve Sciatic-tibial nerve Caudal NAP Caudal CMAP CV (m/s) Amplitude (μV) CV (m/s) Amplitude (mV) CV (m/s) Amplitude (μV) CV (m/s) Amplitude (mV) Control 9 46.3 ± 2.1 23.3 ± 2.6 49.5 ± 1.6 3.9 ± 1.1 60 ± 1.5 101 ± 7 46 ± 0.5 4.9 ± 0.3 Diabetic 9 40.6 ± 1.7 30.9 ± 2.7 46.3 ± 1.0 5.2 ± 1.2 47 ± 1.2 103 ± 8 41.3 ± 0.2 5.8 ± 0.9 P 0.0497 0.0590 0.1176 0.4405 0.0000 0.8557 0.0339 0.3818 -
Data are means ± SE. CMAP, compound muscle action potential; CV, conduction velocity; NAP, nerve action potential.
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- TABLE 2
Morphometric data on L5 DRGs from control and diabetic rats
Identification number Number of cells % Small % Large Ratio of large to small Total Small Large Control 671L 17,888 13,305 4,583 74 26 0.344 672L 16,425 12,519 3,906 76 24 0.312 675L 17,700 13,240 4,460 75 25 0.337 676L 12,033 7,811 4,221 65 35 0.540 677L 14,630 9,529 5,101 65 35 0.535 678L 13,146 8,911 4,235 68 32 0.475 Mean ± SE 15,304 ± 991 10,886 ± 988 4,418 ± 166 71 ± 2 29 ± 2 0.424 ± 0.043 Diabetic 664L 11,587 9,010 2,577 78 22 0.286 665L 13,937 10,590 3,348 76 24 0.316 667L 18,600 15,288 3,312 82 18 0.217 680L 20,218 16,343 3,875 81 19 0.237 681L 12,870 10,442 2,428 81 19 0.233 682L 17,531 12,999 4,532 74 26 0.349 683L 9,188 6,661 2,527 72 28 0.379 Mean ± SE 14,847 ± 1,524 11,619 ± 1,305 3,229 ± 297 78 ± 1 22 ± 1 0.288 ± 0.024 -
Data are means ± SE. Differences (t test) for number of total cells and small cells were not significant (P = 0.814 and 0.672, respectively); differences were significant for number of large cells (P = 0.007), % small (P = 0.014), % large (P = 0.014), and the ratio of large to small (P = 0.015).
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