Acute Hyperglycemia Induces Nitrotyrosine Formation and Apoptosis in Perfused Heart From Rat
Article Figures & Tables
Figures
- FIG. 1.
Coronary perfusion pressure after 2 h infusion with control (11.1 mmol/l d-glucose), high glucose (33.3 mmol/l d-glucose), high glucose plus glutathione (33.3 mmol/l d-glucose plus 0.3 mmol/l glutathione), and osmotic (11.1 mmol/l d-glucose plus 22.2 mmol/l mannitol) solutions. GSH, glutathione.
- FIG. 2.
Nitrotyrosine immmunostaining in heart perfused with control solution (A), high glucose (B), and high glucose plus glutathione (C); negative control (D).
- FIG. 3.
iNOS immunostaining in heart perfused with control solution (A), high glucose (B), and high glucose plus glutathione (C).
- FIG. 4.
Western blot of iNOS in heart perfused with control solution (lane 1), high glucose (lane 2), and high glucose plus glutathione (lane 3).
- FIG. 5.
mRNA of iNOS evaluated by RT-PCR in heart perfused with control solution (lane 1), high glucose (lane 2), and high glucose plus glutathione (lane 3).
- FIG. 6.
NO and superoxide production during 2 h infusion in heart perfused with control solution, high glucose and high glucose plus glutathione. GSH, glutathione.
- FIG. 7.
Apoptosis detected in tissue from heart perfused with control solution (A), high glucose (B), and high glucose plus glutathione (C).
- FIG. 8.
Apoptosis detected by Western blot for caspase 3 (Casp3a) in tissue from heart perfused with control solution (lane 1), high glucose plus glutathione (lane 2), and high glucose (lane 3).
In this Issue
