Glycation Inactivation of the Complement Regulatory Protein CD59

Glycated hCD59 colocalizes with MAC in diabetic human kidneys. Serial sections of the same glomerulus in a diabetic (D) and nondiabetic (ND) kidney stained with anti-MAC or anti–glycated hCD59 antibodies. The arrows indicate positive staining areas for glycated CD59 and MAC in the same glomerulus. (The minor distortion in the anatomy of the glomeruli shown is due to the different planes of the serial sections and the incubation with sodium borohydride before staining with anti–glycated hCD59.) Magnification ×20.