Skip to main content
  • More from ADA
    • Diabetes Care
    • Clinical Diabetes
    • Diabetes Spectrum
    • ADA Standards of Medical Care in Diabetes
    • ADA Scientific Sessions Abstracts
    • BMJ Open Diabetes Research & Care
  • Subscribe
  • Log in
  • My Cart
  • Follow ada on Twitter
  • RSS
  • Visit ada on Facebook
Diabetes

Advanced Search

Main menu

  • Home
  • Current
    • Current Issue
    • Online Ahead of Print
    • ADA Scientific Sessions Abstracts
  • Browse
    • By Topic
    • Issue Archive
    • Saved Searches
    • ADA Scientific Sessions Abstracts
    • Diabetes COVID-19 Article Collection
    • Diabetes Symposium 2020
  • Info
    • About the Journal
    • About the Editors
    • ADA Journal Policies
    • Instructions for Authors
    • Guidance for Reviewers
  • Reprints/Reuse
  • Advertising
  • Subscriptions
    • Individual Subscriptions
    • Institutional Subscriptions and Site Licenses
    • Access Institutional Usage Reports
    • Purchase Single Issues
  • Alerts
    • E­mail Alerts
    • RSS Feeds
  • Podcasts
    • Diabetes Core Update
    • Special Podcast Series: Therapeutic Inertia
    • Special Podcast Series: Influenza Podcasts
    • Special Podcast Series: SGLT2 Inhibitors
    • Special Podcast Series: COVID-19
  • Submit
    • Submit a Manuscript
    • Submit Cover Art
    • ADA Journal Policies
    • Instructions for Authors
    • ADA Peer Review
  • More from ADA
    • Diabetes Care
    • Clinical Diabetes
    • Diabetes Spectrum
    • ADA Standards of Medical Care in Diabetes
    • ADA Scientific Sessions Abstracts
    • BMJ Open Diabetes Research & Care

User menu

  • Subscribe
  • Log in
  • My Cart

Search

  • Advanced search
Diabetes
  • Home
  • Current
    • Current Issue
    • Online Ahead of Print
    • ADA Scientific Sessions Abstracts
  • Browse
    • By Topic
    • Issue Archive
    • Saved Searches
    • ADA Scientific Sessions Abstracts
    • Diabetes COVID-19 Article Collection
    • Diabetes Symposium 2020
  • Info
    • About the Journal
    • About the Editors
    • ADA Journal Policies
    • Instructions for Authors
    • Guidance for Reviewers
  • Reprints/Reuse
  • Advertising
  • Subscriptions
    • Individual Subscriptions
    • Institutional Subscriptions and Site Licenses
    • Access Institutional Usage Reports
    • Purchase Single Issues
  • Alerts
    • E­mail Alerts
    • RSS Feeds
  • Podcasts
    • Diabetes Core Update
    • Special Podcast Series: Therapeutic Inertia
    • Special Podcast Series: Influenza Podcasts
    • Special Podcast Series: SGLT2 Inhibitors
    • Special Podcast Series: COVID-19
  • Submit
    • Submit a Manuscript
    • Submit Cover Art
    • ADA Journal Policies
    • Instructions for Authors
    • ADA Peer Review
Obesity Studies

Adipocyte Lipases and Defect of Lipolysis in Human Obesity

  1. Dominique Langin1,
  2. Andrea Dicker2,
  3. Geneviève Tavernier1,
  4. Johan Hoffstedt2,
  5. Aline Mairal1,
  6. Mikael Rydén2,
  7. Erik Arner3,
  8. Audrey Sicard1,
  9. Christopher M. Jenkins4,
  10. Nathalie Viguerie1,
  11. Vanessa van Harmelen2,
  12. Richard W. Gross4,
  13. Cecilia Holm5 and
  14. Peter Arner2
  1. 1Obesity Research Unit, Institut National de la Santé et de la Recherche Médicale, Université Paul Sabatier (UPS) U586, Louis Bugnard Institute, Toulouse University Hospitals, Paul Sabatier University, Toulouse, France
  2. 2Department of Medicine, Karolinska University Hospital–Huddinge, Stockholm, Sweden
  3. 3Center of Genomics and Bioinformatics, Karolinska Institute, Stockholm, Sweden
  4. 4Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Chemistry, Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri
  5. 5Department of Experimental Medical Science, Division for Diabetes, Metabolism and Endocrinology, Biomedical Center, Lund University, Lund, Sweden
  1. Address correspondence and reprint requests to Dominique Langin, Unité de Recherches sur les Obésités INSERM UPS U586, Institut Louis Bugnard IFR31, BP 84225, 31432 Toulouse Cedex 4, France. E-mail: langin{at}toulouse.inserm.fr
Diabetes 2005 Nov; 54(11): 3190-3197. https://doi.org/10.2337/diabetes.54.11.3190
PreviousNext
  • Article
  • Figures & Tables
  • Suppl Material
  • Info & Metrics
  • PDF
Loading

Article Figures & Tables

Figures

  • FIG. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Selectivity of BAY for inhibition of HSL. A: Concentration-response inhibition by BAY of purified human HSL (hHSL) or rat HSL (rHSL), pancreatic lipase (PL), lipoprotein lipase (LPL), and monoglyceride lipase (MGL) enzymatic activities (n = 3). B: BAY (10−6 mol/l) inhibition of triglyceride hydrolase activity in Cos7 cells expressing human HSL or human ATGL (n = 3). C: Concentration-response inhibition by BAY of mouse adipose tissue extract triglyceride hydrolase activity (n = 3). D: BAY (10−6 mol/l) inhibition of triglyceride hydrolase activity in wild-type and HSL-null mouse adipose tissue extracts (n = 4).

  • FIG. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    Lipolysis in isolated adipocytes from wild-type and HSL-null mice. A: Glycerol release (n = 5). Concentration-response curves of isoprenaline were performed without BAY (squares), with 10−8 mol/l BAY (diamonds), or with 10−6 mol/l BAY (triangles). B: Glycerol release (n = 3). Concentration-response inhibition of BAY was measured on lipolysis induced by 10−4 mol/l isoprenaline. C: FFA release (n = 3). Concentration-response inhibition of BAY was measured on lipolysis induced by 10−4 mol/l isoprenaline.

  • FIG. 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    Effect of BAY on human fat cell lipolysis. A: Antilipolytic effects of BAY (▪, 10−10 mol/l; □, 10−7 mol/l), insulin (•, 10−8 mol/l), and prostaglandin E2 (▵, 10−5 mol/l) on isoprenaline-induced glycerol release. ○, dose-response curve of isoprenaline without antilipolytic agents (n = 4). B: Effect of BAY on basal (□), isoprenaline-induced (○, 10−7 mol/l), and atrial natriuretic peptide–induced (▴, 10−7 mol/l) glycerol release (n = 3). C: Inhibition by BAY (10−5 mol/l) of basal, isoprenaline-induced (Iso, 10−7 mol/l), and atrial natriuretic peptide–induced (ANP, 10−7 mol/l) glycerol and FFA release (n = 6). D: BAY (10−6 mol/l) inhibition of triglyceride (triolein) and diglyceride (MOME) hydrolase activity in human adipose tissue extracts (n = 4).

  • FIG. 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIG. 4.

    HSL and ATGL gene expression in human adipose tissue. HSL and ATGL mRNA levels were quantified by reverse transcription-quantitative PCR and normalized with 18S rRNA levels. A: HSL and ATGL mRNA levels in isolated adipocytes and stromavascular fraction (SVF) from human adipose tissue (n = 6). B: Expression of HSL, ATGL, and peroxisome proliferator–activated receptor γ (PPARγ) mRNA during human preadipocyte differentiation (n = 5). C: Relationship between HSL and ATGL mRNA levels in subcutaneous adipose tissue from 80 obese women. D: Relationship between HSL and ATGL mRNA variation during a hypocaloric diet in subcutaneous adipose tissue from 24 obese women. HSL and ATGL mRNA levels were measured before and after a 10-week hypocaloric diet. The variations are calculated as follows for each individual: (mRNA level after the diet − mRNA level before the diet)/mRNA level before the diet.

  • FIG. 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIG. 5.

    Lipolysis, leptin secretion, and HSL expression in mature adipocytes and differentiated preadipocytes from obese and lean subjects. A: Glycerol and leptin release in isolated mature adipocytes from 42 lean and 81 obese subjects. B: Glycerol and leptin release in preadipocytes differentiated in primary culture from 42 lean and 81 obese subjects. C: Protein expression of HSL and β2-adrenoceptor (β2-AR) in differentiated human preadipocytes from 14 obese and 7 lean subjects determined by Western blot analysis.

  • FIG. 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIG. 6.

    Model for stimulatory pathways in human adipose tissue lipolysis. For the sake of clarity, important nonenzymatic modulators of the lipolytic process such as perilipins are not shown. AC, adenylyl cyclase; AR, adrenoceptor; DG, diglyceride; FA, fatty acid; GC, guanylate cyclase; Gs, stimulatory G protein; MG, monoglyceride; MGL, monoglyceride lipase; NPRA, natriuretic peptide receptor A; PKA, protein kinase A; PKG, protein kinase G; TG, triglyceride.

PreviousNext
Back to top

In this Issue

November 2005, 54(11)
  • Table of Contents
  • Index by Author
Sign up to receive current issue alerts
View Selected Citations (0)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about Diabetes.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Adipocyte Lipases and Defect of Lipolysis in Human Obesity
(Your Name) has forwarded a page to you from Diabetes
(Your Name) thought you would like to see this page from the Diabetes web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Adipocyte Lipases and Defect of Lipolysis in Human Obesity
Dominique Langin, Andrea Dicker, Geneviève Tavernier, Johan Hoffstedt, Aline Mairal, Mikael Rydén, Erik Arner, Audrey Sicard, Christopher M. Jenkins, Nathalie Viguerie, Vanessa van Harmelen, Richard W. Gross, Cecilia Holm, Peter Arner
Diabetes Nov 2005, 54 (11) 3190-3197; DOI: 10.2337/diabetes.54.11.3190

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Add to Selected Citations
Share

Adipocyte Lipases and Defect of Lipolysis in Human Obesity
Dominique Langin, Andrea Dicker, Geneviève Tavernier, Johan Hoffstedt, Aline Mairal, Mikael Rydén, Erik Arner, Audrey Sicard, Christopher M. Jenkins, Nathalie Viguerie, Vanessa van Harmelen, Richard W. Gross, Cecilia Holm, Peter Arner
Diabetes Nov 2005, 54 (11) 3190-3197; DOI: 10.2337/diabetes.54.11.3190
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Keywords

ATGL, adipose triglyceride lipase
BAY, 4-isopropyl-3-methyl-2-[1-(3-(S)-methyl-piperidin-1-yl)-methanoyl]-2H-isoxazol-5–1
FFA, free fatty acid
HSL, hormone-sensitive lipase
KRBA, Krebs-Ringer bicarbonate buffer containing albumin
MOME, 1(3)-monooleoyl-2-0-monooleyl glycerol

Jump to section

  • Article
    • Abstract
    • RESEARCH DESIGN AND METHODS
    • RESULTS
    • DISCUSSION
    • Acknowledgments
    • Footnotes
    • REFERENCES
  • Figures & Tables
  • Suppl Material
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Deficiency of Stat1 in CD11c+ Cells Alters Adipose Tissue Inflammation and Improves Metabolic Dysfunctions in Mice Fed a High-Fat Diet
  • Placental Insulin/IGF-1 Signaling, PGC-1α, and Inflammatory Pathways Are Associated With Metabolic Outcomes at 4–6 Years of Age: The ECHO Healthy Start Cohort
  • Defective FXR-SHP Regulation in Obesity Aberrantly Increases miR-802 Expression, Promoting Insulin Resistance and Fatty Liver
Show more Obesity Studies

Similar Articles

Navigate

  • Current Issue
  • Online Ahead of Print
  • Scientific Sessions Abstracts
  • Collections
  • Archives
  • Submit
  • Subscribe
  • Email Alerts
  • RSS Feeds

More Information

  • About the Journal
  • Instructions for Authors
  • Journal Policies
  • Reprints and Permissions
  • Advertising
  • Privacy Policy: ADA Journals
  • Copyright Notice/Public Access Policy
  • Contact Us

Other ADA Resources

  • Diabetes Care
  • Clinical Diabetes
  • Diabetes Spectrum
  • Scientific Sessions Abstracts
  • Standards of Medical Care in Diabetes
  • BMJ Open - Diabetes Research & Care
  • Professional Books
  • Diabetes Forecast

 

  • DiabetesJournals.org
  • Diabetes Core Update
  • ADA's DiabetesPro
  • ADA Member Directory
  • Diabetes.org

© 2021 by the American Diabetes Association. Diabetes Print ISSN: 0012-1797, Online ISSN: 1939-327X.