Adipose Triglyceride Lipase
Function, Regulation by Insulin, and Comparison With Adiponutrin
Article Figures & Tables
Figures
- FIG. 1.
ATGL and adiponutrin (AN) expression during adipogenesis. ATGL (A) and adiponutrin (B) expression were determined by Q-PCR in 3T3-L1 adipocytes on days 0, 2, 3, 4, 5, and 8 of differentiation (n = 3 per time point). Gene expression was normalized to 18S ribosomal RNA and expressed relative to ATGL expression in day 8 adipocytes.
- FIG. 2.
ATGL and adiponutrin (AN) expression in various murine tissues. ATGL (A) and adiponutrin (B) expression were determined by Q-PCR in BAT, PGAT, subcutaneous adipose tissue (SCAT), mesenteric adipose tissue (MAT), adrenal gland (ADR), testes (TES), soleus (SOL), tibialis anterior (TA), heart (HRT), lung (LNG), kidney (KID), liver (LIV), and hypothalamus (HYP) of 10-week-old male C57BL/6J mice (n = 6 per group). Gene expression was normalized to 18S ribosomal RNA and expressed relative to ATGL expression in BAT.
- FIG. 3.
Overexpression of ATGL in 3T3-L1 adipocytes. Retroviral-mediated overexpression of ATGL in 3T3-L1 adipocytes was achieved via stable transfection with vector alone (L1-Cont) or vector containing full-length ATGL cDNA (L1-ATGL) (three experiments, n = 3 each). ATGL expression (A), basal (B) and stimulated (C) glycerol release, and basal (D) and stimulated (E) NEFA release were then evaluated at day 4 of differentiation. Gene expression was normalized to 18S ribosomal RNA and expressed relative to ATGL expression in the control group. Glycerol and NEFA release were normalized to total cellular protein and expressed relative to the nonstimulated control group. Iso, isoproterenol. *P < 0.05; **P < 0.01; ****P < 0.0001.
- FIG. 4.
Knockdown of ATGL in 3T3-L1 adipocytes. siRNA-mediated knockdown of ATGL in 3T3-L1 adipocytes was achieved by electroporating 3T3-L1 adipocytes with either control siRNA (Cont) or ATGL-specific siRNA (ATGL) at day 4 of differentiation (three experiments, n = 3 each). ATGL expression (A), basal (B) and stimulated (C) glycerol release, and basal (D) and stimulated (E) NEFA release were then evaluated at day 6 of differentiation. Gene expression was normalized to 18S ribosomal RNA and expressed relative to ATGL expression in the control group. Glycerol and NEFA release were normalized to total cellular protein and expressed relative to the nonstimulated control group. Iso, isoproterenol. ***P < 0.001; ****P < 0.0001.
- FIG. 5.
Comparison of siRNA-mediated knockdown of ATGL, adiponutrin (AN), and HSL in fully differentiated 3T3-L1 adipocytes. 3T3-L1 adipocytes were electroporated in the presence of control siRNA or siRNAs specific for ATGL, adiponutrin, or HSL, alone or in combination at day 7 of differentiation (n = 3, representative one of two experiments). ATGL (A), adiponutrin (B), and HSL (C) expression were determined by Q-PCR 48 h later. Gene expression was normalized to 18S ribosomal RNA and expressed relative to ATGL expression in the control group. Basal (D) and stimulated (E) glycerol release and basal (F) and stimulated (G) NEFA release were determined at day 9 of differentiation. Glycerol and NEFA release was normalized to total cell protein and expressed relative to the nonstimulated control group. Iso, isoproterenol. *P < 0.05; **P < 0.01; ***P < 0.001; **** P < 0.0001.
- FIG. 6.
Regulation of ATGL and adiponutrin (AN) expression by nutritional status. ATGL (A) and adiponutrin (B) mRNA expression were determined by Q-PCR in PGAT of 8-week-old male FVB mice that were fed ad libitum (▪), fasted (6, 12, 18, or 24 h) (□), or re-fed (12 or 24 h after a 24-h fast) (□) (n = 10 per group) with the onset of the light cycle (6:00 a.m.) as time 0. Gene expression was normalized to 18S ribosomal RNA and expressed relative to gene expression in ad libitum–fed control mice at time 0.
- FIG. 7.
Regulation of ATGL and adiponutrin (AN) expression by insulin in 3T3-L1 adipocytes. Dose-response curves for ATGL (A) and adiponutrin (B) expression were generated by incubating fully differentiated 3T3-L1 adipocytes in serum-free DMEM supplemented with insulin at the doses indicated for 12 h (n = 3, representative one of three experiments). Data were normalized to 18S ribosomal RNA and expressed relative to gene expression in control cells incubated without insulin. Time course for ATGL (C) and adiponutrin (D) expression were generated by incubating fully differentiated 3T3-L1 adipocytes in either DMEM containing 10% FBS or serum-free DMEM (serum starved) with and without insulin at 1,000 ng/ml for ATGL or 1 ng/ml for adiponutrin for the time indicated (n = 3, representative one of three experiments). ▪, FBS; •, serum starved; ⧫, FBS plus insulin; ▴, serum starved plus insulin. Data were normalized to 18S and expressed relative to gene expression in the control group at time 0. To convert ng/ml to nmol/l, multiply by 0.174.
- FIG. 8.
ATGL and adiponutrin (AN) expression in adipose tissue of STZ-administered mice. ATGL (A) and adiponutrin (B) expression were determined by Q-PCR in PGAT of ad libitum–fed, 10-week-old male mice given vehicle (control), STZ, or STZ followed by insulin replacement for 24 h (STZ + Ins) (n = 8–10 per group). Data were normalized to HPRT and expressed relative to gene expression in the control group. *P < 0.05; ****P < 0.0001.
- FIG. 9.
ATGL and adiponutrin (AN) expression in adipose tissue of FIRKO mice. ATGL (A) and adiponutrin (B) expression were determined by Q-PCR in PGAT of 2-year-old, ad libitum–fed, male FIRKO mice (n = 4 per group). Data were normalized to HPRT and expressed relative to gene expression in the control group. *P < 0.05.
