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Original Article

Inactivation of GSK-3β by Metallothionein Prevents Diabetes-Related Changes in Cardiac Energy Metabolism, Inflammation, Nitrosative Damage, and Remodeling

  1. Yuehui Wang1,2,
  2. Wenke Feng1,2,
  3. Wanli Xue2,
  4. Yi Tan1,2,3,
  5. David W. Hein4,
  6. Xiao-Kun Li1,5 and
  7. Lu Cai1,2,3,4
  1. 1Chinese-American Research Institute for Diabetic Complications, Wenzhou Medical College, Zhejiang, China;
  2. 2Department of Medicine, University of Louisville, Louisville, Kentucky;
  3. 3Department of Pediatrics, University of Louisville, Louisville, Kentucky;
  4. 4Department of Pharmacology and Toxicology, University of Louisville, Louisville, Kentucky;
  5. 5Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Jilin, China.
  1. Corresponding authors: Lu Cai, l0cai001{at}louisville.edu, and Xiao-Kun Li, xiaokunli{at}163.net.
Diabetes 2009 Jun; 58(6): 1391-1402. https://doi.org/10.2337/db08-1697
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  • FIG. 1.
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    FIG. 1.

    Diabetic hyperglycemia and activation of GSK-3β. Before mice were killed at the indicated times, blood glucose was monitored at indicated times, using tail-vein blood (A), and cardiac tissues were used for Western blotting analysis of total and phosphorylated GSK-3β (B). *P < 0.05 vs. corresponding controls. Ms, months; Ws, weeks; WT, wild type.

  • FIG. 2.
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    FIG. 2.

    Diabetes-induced glycogen synthase (GS) phosphorylation and decrease of HK II expression. Cardiac tissues were collected, as indicated in Fig. 1, for detecting total and phosphorylated glycogen synthase (Ser641) (A) and HK II expression (B) by Western blotting. *P < 0.05 vs. control. Ms, months; Ws, weeks; WT, wild type.

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    FIG. 3.

    Diabetes-increased PPARα expression and lipid accumulation. Cardiac tissues were collected, as indicated in Fig. 1, for detecting PPARα (A) and PGC-1α (B) expressions by Western blotting and cardiac lipid accumulation by Oil Red O staining (400×) (C) and triglyceride measurement (D). Panel D presents the data only from diabetic mice 2 weeks (Ws) after diabetes. *P < 0.05 vs. control; #P < 0.05 vs. wild-type (WT) diabetic group. Ms, months. (A high-quality digital representation of this figure is available in the online issue.).

  • FIG. 4.
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    FIG. 4.

    Diabetes-induced cardiac inflammation. Real-time RT-PCR was used to examine the expression of TNF-α (A), ICAM-1 (B), and PAI-1 mRNA (C) in the hearts of diabetic mice at indicated postdiabetic times. TNF-α, ICAM-1, and PAI-1 expressions were normalized to GAPDH, by which the fold changes in expression were calculated using 2−ΔΔCt method. Expression of PAI-1 was further confirmed for its protein level by Western blotting (D) and localization by immunohistochemical staining (E). *P < 0.05 vs. corresponding controls. Ms, months; Ws, weeks; WT, wild type. (A high-quality digital representation of this figure is available in the online issue.)

  • FIG. 5.
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    FIG. 5.

    FFA-induced upregulation of inflammatory cytokines. Cultured cardiac (H9c2) cells were directly exposed to TNF-α at a concentration of 1.0 ng/ml for 24 h, which did not increase intracellular triglyceride levels (A). Direct exposure of cardiac cells to FFA (palmitate) at 50 μmol/l for 24 h significantly increased intracellular triglyceride levels (A) and also significantly increased mRNA expression of inflammatory cytokines TNF-α (B) and PAI-1 (C). *P < 0.05 vs. control.

  • FIG. 6.
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    FIG. 6.

    Diabetes-increased cardiac nitrosative damage and fibrosis. Cardiac nitrosative damage was examined by 3-NT accumulation with Western blotting analysis (A). Cardiac fibrosis was examined by Western blotting analysis of CTGF expression (B) and Sirius Red staining of collagen (C). *P < 0.05 vs. corresponding controls. Ms, months; Ws, weeks; WT, wild type. (A high-quality digital representation of this figure is available in the online issue.)

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    FIG. 7.

    Inhibition of GSK-3β by its inhibitor attenuated diabetes-induced changes related to glucose and lipid metabolism. Once diabetes was diagnosed on day 3 after mice were injected with STZ, half of these diabetic mice were immediately administered GSK-3β–specific inhibitor SB216763 at 600 μg/kg every other day for 2 months, and then cardiac glycogen synthase (GS) phosphorylation (A), HK II expression (B), PPARα expression (C), and PGC-1α expression (D) were examined by Western blotting. Cardiac lipid accumulation was examined by Oil Red O staining (400×) (E). *P < 0.05 vs. corresponding controls; #P < 0.05 vs. corresponding diabetes. (A high-quality digital representation of this figure is available in the online issue.)

  • FIG. 8.
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    FIG. 8.

    Inhibition of GSK by its inhibitor attenuated diabetes-induced cardiac inflammation, nitrosative damage, and fibrosis. Experimental approaches and cardiac tissue sampling are the same as those in Fig. 6. Samples were examined for cardiac expression of PAI-1 by real-time RT-PCR (A) and Western blotting (B), cardiac 3-NT accumulation (C), and cardiac fibrosis by Western blotting for CTGF expression (D) and Sirius Red staining of collagen (200×) (E). *P < 0.05 vs. corresponding controls; #P < 0.05 vs. corresponding diabetes. F: schematic illustration of the mechanisms by which metallothionein (MT) preserves GSK-3β phosphorylation to maintain glucose and lipid metabolism balance under diabetic conditions and, consequently, inhibit cardiac lipid accumulation, inflammation, oxidative/nitrosative damage, and remodeling. Solid lines present the experimental finding from the present study, and dashed lines indicate well-known pathways from the literature. GS, glycogen synthase; IR, insulin receptor. (A high-quality digital representation of this figure is available in the online issue.)

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Inactivation of GSK-3β by Metallothionein Prevents Diabetes-Related Changes in Cardiac Energy Metabolism, Inflammation, Nitrosative Damage, and Remodeling
Yuehui Wang, Wenke Feng, Wanli Xue, Yi Tan, David W. Hein, Xiao-Kun Li, Lu Cai
Diabetes Jun 2009, 58 (6) 1391-1402; DOI: 10.2337/db08-1697

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Inactivation of GSK-3β by Metallothionein Prevents Diabetes-Related Changes in Cardiac Energy Metabolism, Inflammation, Nitrosative Damage, and Remodeling
Yuehui Wang, Wenke Feng, Wanli Xue, Yi Tan, David W. Hein, Xiao-Kun Li, Lu Cai
Diabetes Jun 2009, 58 (6) 1391-1402; DOI: 10.2337/db08-1697
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