Targeting BCAA Catabolism to Treat Obesity-Associated Insulin Resistance

The BCAA catabolic pathway shows a strong correlation with IR in a mouse population. A: Comparison of correlation strengths of the expression of BCAA pathway genes, non-BCAA amino acid pathway genes, and all genes with IR-related traits in mice. The correlation data between tissue-specific expression profiling and measurements of clinical traits were extracted from the HMDP, which contains ∼100 strains of genetically divergent mice fed an HFD. The mean and SE of the absolute values of Pearson correlations between each gene within a gene category (BCAA, non-BCAA, all genes) and a trait are shown. The significance of differences in the mean correlation strength between gene categories was calculated by using the Student t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. B: Correlation of individual BCAA catabolic genes in different tissues for fasting glucose, fasting insulin, and HOMA-IR in HMDP mice fed an HFD. Red indicates a positive correlation, whereas blue indicates a negative correlation. *P < 0.05 and **P < 0.05 after Bonferroni correction for the number of genes and traits.

Systematic downregulation of the BCAA catabolic pathway leads to a BCAA catabolic defect in ob/ob mice. A: Quantitative PCR results of BCAA catabolic genes in white adipose tissue, skeletal muscle, and liver in lean wild-type (WT) mice (n = 4) and ob/ob mice (n = 4) deprived of food for 6 h. B: Illustration of the partial BCAA catabolic process with enzymes, intermediates, and derivatives. C: Relative levels of BCAAs and their metabolites in plasma and tissues of lean WT mice (n = 6–8) and ob/ob mice (n = 8). Male mice, age 14 weeks, were deprived of food for 6 h before being sacrificed. D: Serum concentrations of BCAAs and BCKAs in lean WT mice (n = 8) and ob/ob mice (n = 10). Male mice, age 8 weeks, were deprived of food from 8:00 a.m. to 5:00 p.m. and then supplied with chow diet for 1 hour. Blood (100 μL) was collected at 6:00 p.m. from the orbital sinus by using capillary tubes for serum collection. *P < 0.05 and **P < 0.01 vs. WT mice. ΚΙC, α-ketoisocaproic acid; KIV, α-ketoisovaleric acid; KMV, α-keto-β-methylvaleric acid.

The BCKDK inhibitor restores BCAA catabolism and attenuates IR in ob/ob mice. ob/ob mice were treated with the vehicle (Ctrl) or BT2 (at 10 weeks [A, B, and E] or 4–6 weeks [C and D]) by oral gavage (n = 6 mice in each group). We analyzed BCKD activity in various tissues (A), fasting plasma levels of BCAAs (B, left) and BCKAs (B, right), glucose tolerance test results (C), insulin tolerance test results (D), fasting plasma insulin level (E), body weight (F), and food intake (G). *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.0001 vs. Ctrl. ΚΙC, α-ketoisocaproic acid; KIV, α-ketoisovaleric acid; KMV, α-keto-β-methylvaleric acid; WAT, white adipose tissue.

Altering BCAA intake affects IR in ob/ob mice. ob/ob mice were fed an NPD (20% protein by weight) or an LPD (6% protein by weight) for 4 weeks beginning at 10 weeks of age. For the BCAA group (LPD + BCAA), supplementation of BCAAs in drinking water (3 mg/mL) was started after mice had eaten the LPD for 2 weeks and lasted 2 weeks. We analyzed fasting plasma levels of BCAAs and metabolites (n = 8 mice; *P < 0.05 vs. NPD; &P < 0.05 vs. LPD) (A), glucose tolerance test results (n = 6–13 mice; **P < 0.01, LPD vs. NPD; &P < 0.05, LPD vs. LPD + BCAA) (B), insulin tolerance test results (n = 6 mice in each group; *P < 0.05, ***P < 0.001, LPD vs. NPD; &P < 0.05, LPD vs. LPD + BCAA) (C), fasting plasma insulin levels (n = 8–12 mice; *P < 0.05; &P < 0.05) (D), and body weight (n = 14 mice in each group; **P < 0.01).

BCAAs and BCKAs contribute to impaired insulin signaling in ob/ob mice. A–C: Representative immunoblots for specific proteins, created by using tissue lysates from skeletal muscle, liver, and white adipose tissue of mice without or with insulin (Ins) injection for 10 min following 6 h of food deprivation. ob/ob mice were fed an NPD (20% protein by weight) or an LPD (6% protein by weight) for 4 weeks beginning at 10 weeks of age (A). BCAA supplementation (LPD + BCAA or LPD/amino acids) in drinking water (3 mg/mL) was started after mice had consumed the LPD for 2 weeks and lasted 2 weeks (B). ob/ob mice were treated with the vehicle (Ctrl) or BT2 by oral gavage for 5 weeks (C). The graphs below the immunoblots in A–C present densitometric values of the bands. D–F: Representative immunoblots for specific proteins, created by using cell lysates. 3T3-L1 cells were treated with FBS- and BCAA-free DMEM for 1–2 h before BCAA (500 μmol/L), BCKA (500 μmol/L), or rapamycin (100 nmol/L) treatment for 1 h (D and F) or various times (E), followed by insulin treatment (10 nmol/L) for 1 h (D). *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.0001.