PT  - JOURNAL ARTICLE
AU  - Bierhaus, Angelika
AU  - Chevion, Shlomit
AU  - Chevion, Mordechai
AU  - Hofmann, Marion
AU  - Quehenberger, Peter
AU  - Illmer, Thomas
AU  - Luther, Thomas
AU  - Berentshtein, Eduard
AU  - Tritschler, Hans
AU  - Müller, Martin
AU  - Wahl, Peter
AU  - Ziegler, Reinhard
AU  - Nawroth, Peter P
TI  - Advanced Glycation End Product-Induced Activation of NF-κB is Suppressed by α-Lipoic Acid in Cultured Endothelial Cells
AID  - 10.2337/diab.46.9.1481
DP  - 1997 Sep 01
TA  - Diabetes
PG  - 1481--1490
VI  - 46
IP  - 9
4099  - http://diabetes.diabetesjournals.org/content/46/9/1481.short
4100  - http://diabetes.diabetesjournals.org/content/46/9/1481.full
SO  - Diabetes1997 Sep 01; 46
AB  - Depletion of cellular antioxidant defense mechanisms and the generation of oxygen free radicals by advanced glycation end products (AGEs) have been proposed to play a major role in the pathogenesis of diabetic vascular complications. Here we demonstrate that incubation of cultured bovine aortic endothelial cells (BAECs) with AGE albumin (500 nmol/l) resulted in the impairment of reduced glutathione (GSH) and ascorbic acid levels. As a consequence, increased cellular oxida-tive stress led to the activation of the transcription factor NF-KB and thus promoted the upregulation of various NF-KB-controlled genes, including endothelial tissue factor. Supplementation of the cellular antiox-idative defense with the natural occurring antioxidant α-lipoic acid before AGE albumin induction completely prevented the AGE albumin–dependent depletion of reduced glutathione and ascorbic acid. Electrophoretic mobility shift assays (EMSAs) revealed that AGE albumin-mediated NF-KB activation was also reduced in a time- and dose-dependent manner as long as α-lipoic acid was added at least 30 min before AGE albumin stimulation. Inhibition was not due to physical interactions with protein DNA binding, since α-lipoic acid, directly included into the binding reaction, did not prevent binding activity of recombinant NF-KB. Western blots further demonstrated that α-lipoic acid inhibited the release and translocation of NF-KB from the cytoplasm into the nucleus. As a consequence, α-lipoic acid reduced AGE albumin-induced NF-KB mediated transcription and expression of endothelial genes relevant in diabetes, such as tissue factor and endothelin-1. Thus, supplementation of cellular antioxidative defense mechanisms by extracellularly administered α-lipoic acid reduces AGE albumin-induced endothelial dysfunction in vitro.