RT Journal Article
SR Electronic
T1 Insulin Secretion and Glucose Kinetics During Exercise With and Without Pharmacological α<sub>1</sub>- and α<sub>2</sub>-Receptor Blockade
JF Diabetes
JO Diabetes
FD American Diabetes Association
SP 1834
OP 1843
DO 10.2337/diabetes.50.8.1834
VO 50
IS 8
A1 Aarnio, Pauliina
A1 Lauritsen, Torsten
A1 Dela, Flemming
YR 2001
UL http://diabetes.diabetesjournals.org/content/50/8/1834.abstract
AB The mechanism behind exercise-induced decreases in plasma insulin concentrations was examined in eight healthy young men. In addition, the influence of specific α1- and α2-adrenoceptor blockade on glucose kinetics during exercise was studied. To test the hypothesis that exercise-induced decreases in insulin secretion are mediated via α2-adrenoceptors, all subjects exercised for 60 min on separate occasions under four conditions: with and without α1-receptor blockade (1 mg prazosin) and with and without or α2-receptor blockade (15 mg yohimbine). Glucose kinetics were measured using [3-3H]glucose. During exercise with α2-receptor blockade, the insulin concentration initially increased (first 20 min) then decreased, whereas it continually decreased in the corresponding control experiment. The C-peptide concentration did not change during exercise with α2-receptor blockade but decreased in the control experiment. During exercise with α1-receptor blockade and corresponding control experiments, insulin and C-peptide levels always decreased. With α1-receptor blockade, the glucose concentration increased (first 30 min) and then decreased, whereas it slightly decreased in all other experiments. In addition, with α1-receptor blockade, the glucose rate of appearance (Ra) increased rapidly (because of higher catecholamine concentrations in α1-receptor blockade versus control) and the glucose rate of disappearance (Rd) was higher compared with control. During exercise with α2-receptor blockade, the Ra and Rd were always lower compared with control. Therefore, we conclude that exercise-induced decreases in insulin secretion are mediated via α2-adrenoceptors and that blockade of α1- and α2-adrenoceptors during exercise elicits opposite responses in glucose Ra and Rd.