RT Journal Article
SR Electronic
T1 Expression of Adiponectin Receptor mRNA in Human Skeletal Muscle Cells Is Related to In Vivo Parameters of Glucose and Lipid Metabolism
JF Diabetes
JO Diabetes
FD American Diabetes Association
SP 2195
OP 2201
DO 10.2337/diabetes.53.9.2195
VO 53
IS 9
A1 Staiger, Harald
A1 Kaltenbach, Simone
A1 Staiger, Katrin
A1 Stefan, Norbert
A1 Fritsche, Andreas
A1 Guirguis, Alke
A1 Péterfi, Claudia
A1 Weisser, Melanie
A1 Machicao, Fausto
A1 Stumvoll, Michael
A1 Häring, Hans-Ulrich
YR 2004
UL http://diabetes.diabetesjournals.org/content/53/9/2195.abstract
AB The adiponectin receptors, AdipoR1 and AdipoR2, are thought to transmit the insulin-sensitizing, anti-inflammatory, and atheroprotective effects of adiponectin. In this study, we examined whether AdipoR mRNA expression in human myotubes correlates with in vivo measures of insulin sensitivity. Myotubes from 40 metabolically characterized donors expressed 1.8-fold more AdipoR1 than AdipoR2 mRNA (588 ± 35 vs. 321 ± 39 fg/μg total RNA). Moreover, the expression levels of both receptors correlated with each other (r = 0.45, P < 0.01). AdipoR1 mRNA expression was positively correlated with in vivo insulin and C-peptide concentrations, first-phase insulin secretion, and plasma triglyceride and cholesterol concentrations before and after adjustment for sex, age, waist-to-hip ratio, and body fat. Expression of AdipoR2 mRNA clearly associated only with plasma triglyceride concentrations. In multivariate linear regression models, mRNA expression of AdipoR1, but not AdipoR2, was a determinant of first-phase insulin secretion independent of insulin sensitivity and body fat. Finally, insulin did not directly modify myotube AdipoR1 mRNA expression in vitro. In conclusion, we provide evidence that myotube mRNA levels of both receptors are associated with distinct metabolic functions but not with insulin sensitivity. AdipoR1, but not AdipoR2, expression correlated with insulin secretion. The molecular nature of this link between muscle and β-cells needs to be further clarified.