RT Journal Article
SR Electronic
T1 The Genetic Program of Pancreatic β-Cell Replication In Vivo
JF Diabetes
JO Diabetes
FD American Diabetes Association
SP 2081
OP 2093
DO 10.2337/db16-0003
VO 65
IS 7
A1 Klochendler, Agnes
A1 Caspi, Inbal
A1 Corem, Noa
A1 Moran, Maya
A1 Friedlich, Oriel
A1 Elgavish, Sharona
A1 Nevo, Yuval
A1 Helman, Aharon
A1 Glaser, Benjamin
A1 Eden, Amir
A1 Itzkovitz, Shalev
A1 Dor, Yuval
YR 2016
UL http://diabetes.diabetesjournals.org/content/65/7/2081.abstract
AB The molecular program underlying infrequent replication of pancreatic β-cells remains largely inaccessible. Using transgenic mice expressing green fluorescent protein in cycling cells, we sorted live, replicating β-cells and determined their transcriptome. Replicating β-cells upregulate hundreds of proliferation-related genes, along with many novel putative cell cycle components. Strikingly, genes involved in β-cell functions, namely, glucose sensing and insulin secretion, were repressed. Further studies using single-molecule RNA in situ hybridization revealed that in fact, replicating β-cells double the amount of RNA for most genes, but this upregulation excludes genes involved in β-cell function. These data suggest that the quiescence-proliferation transition involves global amplification of gene expression, except for a subset of tissue-specific genes, which are “left behind” and whose relative mRNA amount decreases. Our work provides a unique resource for the study of replicating β-cells in vivo.