RT Journal Article
SR Electronic
T1 RNA Binding Protein Ybx2 Regulates RNA Stability During Cold-Induced Brown Fat Activation
JF Diabetes
JO Diabetes
FD American Diabetes Association
SP 2987
OP 3000
DO 10.2337/db17-0655
VO 66
IS 12
A1 Xu, Dan
A1 Xu, Shaohai
A1 Kyaw, Aung Maung Maung
A1 Lim, Yen Ching
A1 Chia, Sook Yoong
A1 Chee Siang, Diana Teh
A1 Alvarez-Dominguez, Juan R.
A1 Chen, Peng
A1 Leow, Melvin Khee-Shing
A1 Sun, Lei
YR 2017
UL http://diabetes.diabetesjournals.org/content/66/12/2987.abstract
AB Recent years have seen an upsurge of interest in brown adipose tissue (BAT) to combat the epidemic of obesity and diabetes. How its development and activation are regulated at the posttranscriptional level, however, has yet to be fully understood. RNA binding proteins (RBPs) lie in the center of posttranscriptional regulation. To systemically study the role of RBPs in BAT, we profiled >400 RBPs in different adipose depots and identified Y-box binding protein 2 (Ybx2) as a novel regulator in BAT activation. Knockdown of Ybx2 blocks brown adipogenesis, whereas its overexpression promotes BAT marker expression in brown and white adipocytes. Ybx2-knockout mice could form BAT but failed to express a full thermogenic program. Integrative analysis of RNA sequencing and RNA-immunoprecipitation study revealed a set of Ybx2’s mRNA targets, including Pgc1α, that were destabilized by Ybx2 depletion during cold-induced activation. Thus, Ybx2 is a novel regulator that controls BAT activation by regulating mRNA stability.