Table 1

Cell surface markers to distinguish between KCs and RHMs*

SymbolDescriptionNCHFD
KCRHMf(KC/RHM)KCRHMf(KC/RHM)
KC marker
 F8Coagulation factor VIII20,3554,238512,0741,19810
 KitKit oncogene11,3002,18655,43049211
 Wnt2Wingless-related MMTV integration site 24,83681862,58023311
 PrelpProline arginine-rich end leucine-rich repeat3,48569952,38323410
 Masp1Mannan-binding lectin serine peptidase 12,39645051,91518211
 VwfVon Willebrand factor homolog2,32041667871217
 Wnt9bWingless-type MMTV integration site 9B1,14915672882114
 SelpSelectin, platelet97317851,0068712
 Cdh13Cadherin 1392911489826415
 Dkk3Dickkopf homolog 3 (Xenopus laevis)9111566447499
 TnxbTenascin XB82815455774114
 Col3a1Collagen, type III, α 175210087114615
 Fbln2Fibulin 23556163973212
 PdgfdPlatelet-derived growth factor, D polypeptide3436652312310
 Col1a2Collagen, type I, α 23815963792913
 Col1a1Collagen, type I, α 13575074232021
RHM marker
 C1qaComplement component 1, q subcomponent, α polypeptide15,63580,760539,139107,3293
 C1qcComplement component 1, q subcomponent, C chain15,45974,157534,12998,7103
 Apoc1Apolipoprotein C-I2,51012,32552,73410,2364
 C4bComplement component 4B (Chido blood group)2,3617,77332,7988,6563
 Gbp2bGuanylate binding protein 2b1,1565,56953,82413,6254
 C6Complement component 69278,11891,1426,3036
 Kif23Kinesin family member 239122,63331,2573,4363
 CpqCarboxypeptidase Q6612,88441,5714,2433
 C4aComplement component 4A (Rodgers blood group)22186942247803
 KcpKielin/chordin-like protein754716632314
 Slc1a3Solute carrier family 1 (glial high affinity glutamate transporter), member 3362206722283
  • *Differentially expressed genes between KC and RHMs that were classified within the GO cellular component term “extracellular region part” are listed.

  • †The top 16 KC marker genes listed are expressed at least fivefold higher in KC vs. RHM from NC- or HFD-fed mice. The top 11 RHM markers are all expressed at least threefold higher in RHMs vs. KCs. All markers are expressed at 200 counts per gene or more. RNA-seq studies used three biological replicates per each group.