Table 2

Chemical features and VEGF binding activity of K5 derivatives

GAGSO3/COO ratioAverage molecular weight (Da)VEGF-mediated cell-cell adhesion ID50 (µg/mL)SPR
VEGF/heparin binding ID50 (µg/mL)VEGF/sVEGFR2 binding ID50 (µg/mL)
K5030,000No inhibitionNo inhibitionNo inhibition
K5-NS1.0015,000>1004.512.0
K5-N,OS(L)1.7013,00010.25.09.5
K5-N,OS(H)3.7715,0000.50.0050.2
Heparin2.1413,6000.20.41.0
  • Sulfate/carboxyl ratio (SO3/COO) and molecular weight values are from Leali et al. (28). GAGs were assessed for the capacity to inhibit the formation of HSPG/VEGF/VEGFR2 ternary complexes in a VEGF-mediated cell-cell adhesion assay and to prevent the binding of VEGF to heparin and sVEGFR2 immobilized to Biacore sensor chips.