Table 3

Pathways significantly changed in the obese compared with the lean co-twin (IPA of the differentially expressed genes, n = 26 discordant pairs)

Ingenuity canonical pathwaysP valueRegulationMolecules
OXPHOS<0.0001DownregulatedSDHB, COX6C, COX5B, NDUFB5, COX8A, ATP5L, ATP5G2, NDUFB8, ATP5S, NDUFA2, NDUFB10, NDUFS1, NDUFAB1, ATPAF1, ATP5I, COX4I1, NDUFA8, NDUFS4, ATP5O, ATP5A1, COX7C, NDUFS3, UQCRB, ATP5C1, COX11, NDUFB11, ATP5B, NDUFA6, CYC1, UQCRC2, COX7A2, NDUFA12, SDHD, UQCRC1
Valine degradation I<0.0001DownregulatedHADHB, HIBADH, BCKDHA, AUH, ACAD8, ACADSB, DBT, ALDH6A1, HADHA, BCKDHB
Glutaryl-CoA degradation<0.0001DownregulatedHADHB, ACAT2, ACAT1, HADHA, HADH, GCDH, HSD17B8
Isoleucine degradation I<0.0001DownregulatedHADHB, ACAT2, AUH, ACAD8, ACAT1, ACADSB, HADHA
Ketogenesis<0.0001DownregulatedHADHB, BDH1, ACAT2, ACAT1, HMGCL, HADHA
FAO I<0.0001DownregulatedHADHB, ECI2, AUH, ACADM, ECI1, HADHA, HADH, HSD17B8
Tryptophan degradation III (eukaryotic)<0.0001DownregulatedHADHB, ACAT2, ACAT1, HADHA, HADH, GCDH, HSD17B8
Ketolysis<0.0001DownregulatedHADHB, BDH1, ACAT2, ACAT1, HADHA
TCA II (eukaryotic)<0.0001DownregulatedSDHB, DHTKD1, SUCLG1, DLST, SDHD, FH, IDH3B
Leucine degradation I<0.0001DownregulatedAUH, MCCC1, HMGCL, ACADM, MCCC2