Table 1

Insulitis frequency in donors

Case IDSections (n)Ins+ CD3Ins+ CD3+Ins CD3+Ins CD3Total islets*Insulitis frequency (%)Age at onset (years)Diabetes duration (years)Age at demise (years)Autoantibody results§
T1D626467301,0171,0270.33.009.0012.00Negative
6212125113101,6011,6751.415.005.0020.00
6268501138088221.79.003.0012.00
626568655085272.13.008.0011.00GADA+
603940253243312.116.7012.0028.70GADA+ IA-2A+ ZnT8A+
6046592262683682.210.808.0018.80IA-2A+ ZnT8A+
611314212891,6401,6982.211.521.5813.10
622483024121,4861,5522.319.501.5021.00Negative
6088773764335192.526.205.0031.20GADA+ IA-2A+ ZnT8A+
624561317281,0211,1872.915.007.0022.00GADA+ IA-2A+
624767720251,0681,1903.823.400.6024.00
61951602531,3851,4403.814.205.0019.20GADA+ IA-2A+ ZnT8A+
60704561583334125.615.607.0022.60IA-2A+
6198216951812116.619.003.0022.00GADA+ IA-2A+ ZnT8A+
605291027419219996.811.001.0012.00IA-2A+
6209113242541,2471,3757.04.750.255.00IA-2A+ ZnT8A+
6243816255328591,1087.98.005.0013.00
622815250246742,4473,01710.613.000.0013.00GADA+ IA-2A+ ZnT8A+
Total1441,01650938617,54719,458
AAb+619751,98128042,0131.422.00GADA+ IA-2A+
6267132,1491398182,3146.423.00GADA+ IA-2A+
Total184,1301678224,327
  • Multiple slide sections were reviewed from each donor pancreas, and islets were counted by insulin immunopositivity (Ins+, Ins) and insulitis status (CD3, CD3+). The total number of insulitic islets divided by the total number of islet was used to calculate insulitis frequency (%) for each donor. Donors were listed in order of increasing insulitis frequency (%). Demographic information for ages at demise and diabetes onset, diabetes duration, and autoantibody types were also shown.

  • *Total islets calculated as the sum of four islet subtypes.

  • †Insulitis frequency (%) calculated as sum insulitic islets: (Ins+ CD3+ and Ins CD3+)/total islets.

  • ‡Calculated as age at demise minus diabetes duration.

  • §All donors with insulitis had autoantibody testing by radioimmunoassay. Radioimmunoassay data values were converted to National Institute of Diabetes and Digestive and Kidney Diseases units and defined as positive if one or more of the following applied: GADA if ≥20, IA-2A if ≥5, or ZnT8A if ≥0.020.

  • ‖IAA were not listed for donors with T1D as they could not be distinguished as autoantibodies versus antibodies to parenteral insulin as described in research design and methods. An empty field indicates that only insulin autoantibodies were detected by radioimmunoassay.

  • ¶AAb+ donors without diabetes.