Table 1

List of primary antibodies used in this study

Primary antibodyManufacturerDilution
IRSanta Cruz Biotechnology, sc-7111/400
IGF1RSanta Cruz Biotechnology, sc-7131/400
Catalytic subunits of phosphoinositide 3-kinase (p110α/β)Cell Signaling Technology, 4249, 30111/1,000
Regulatory subunits of phosphoinositide 3-kinase (p85α)Millipore, 06-1951/5,000 in 1% milk
Kinase AktCell Signaling Technology, 92721/1,000
pAkt Thr308Cell Signaling Technology, 92751/1,000
pAkt Ser473Cell Signaling Technology, 92711/1,000
Glycogen synthase kinase 3 (GSK3)Cell Signaling Technology, 93151/1,000
pGSK3 Ser21/9Cell Signaling Technology, 93311/1,000
Ribosomal S6KCell Signaling Technology, 27081/1,000
pS6K Thr389Cell Signaling Technology, 92341/1,000
Eukaryotic translocation initiation factor 4 binding protein (4EBP)Cell Signaling Technology, 96441/1,000
p4EBP Ser65Cell Signaling Technology, 94511/1,000
MAPKCell Signaling Technology, 46951/1,000
pMAPK Thr202/Tyr204Cell Signaling Technology, 43701/1,000
LPLAbcam, 213561/1,000
SREBPAbcam, 32591/200
Fatty acid synthaseCell Signaling Technology, 31801/1,000
PPARαAbcam, 89341/1,500
PPARγSanta Cruz Biotechnology, sc-72731/200
FATP1Santa Cruz Biotechnology, sc-319551/400