TABLE 3

Analysis of single-channel properties

ConditionConfigurationτburst (ms)τclosed (ms)POn
Intact cellCell-attached31 ± 6427 ± 930.09 ± 0.0312
100 μmol/l MgATPInside-out13 ± 3489 ± 1550.06 ± 0.038
100 μmol/l MgADP + 100 μmol/l MgATPInside-out35 ± 12221 ± 560.16 ± 0.059
100 μmol/l MgATPOpen cell39 ± 899 ± 380.21 ± 0.027
100 μmol/l ATP, Mg2+ freeOpen cell18 ± 4500 ± 1360.05 ± 0.025
  • All experiments were carried out on β-cells, using the configurations and internal solutions indicated. τburst, mean burst duration; τclosed, mean interburst interval.