TABLE 1

Colony-forming efficiency and replicative potential of hTERT preadipocyte subtypes

Abdominal subcutaneousMesentericOmental
Colony-forming efficiency (%)68.2 ± 2.268.4 ± 1.867.2 ± 3.0
Rapidly replicating subtype cells (%)25 ± 5*8 ± 34 ± 2
Slowly replicating subtype cells (%)75 ± 5*92 ± 396 ± 2
Modal number of doublings achieved by rapidly replicating subtype cells9.7 ± 0.59.5 ± 0.29.3 ± 0.2
Modal number of doublings achieved by slowly replicating subtype cells4.3 ± 0.24.5 ± 0.24.0 ± 0.3
  • Data are means ± SE. Abdominal subcutaneous, mesenteric, and omental hTERT strains (six strains from each depot [S2.5, S2.11, S2.13, S3.1, S3.5, S3.8, M2.5, M2.6, M2.10, M3.1, M3.5, M3.8, Om2.2, Om2.5, Om2.7, Om3.1, Om3.5, and Om3.8]) were cloned by plate dilution. Fifty cells from each depot were plated into each of 10 96-well plates. Twenty-one days after cloning, cell numbers were determined. Colony-forming efficiency is expressed as percent of cells plated that formed colonies.

  • * Denotes significant difference from other values in the same row (P < 0.05; Duncan’s multiple range test). Colony-forming efficiencies did not differ significantly among depots (P = 0.92; ANOVA).