Activity of enzymes of fatty acid utilization and oxidative capacity in standard diet–and fat-fed mice
| 5 weeks | 20 weeks | |||
|---|---|---|---|---|
| Standard diet | Fat | Standard diet | Fat | |
| Citrate synthase | 20.0 ± 1.6 | 25.0 ± 0.6* | 16.9 ± 1.9 | 26.5 ± 1.9† |
| βHAD | 2.40 ± 0.18 | 3.19 ± 0.11† | 2.27 ± 0.28 | 3.82 ± 0.31† |
| MCAD | 0.75 ± 0.05 | 1.12 ± 0.04† | 0.63 ± 0.10 | 1.40 ± 0.14† |
| CPT-1 | 0.36 ± 0.05 | 0.49 ± 0.04* | 0.30 ± 0.02 | 0.47 ± 0.07* |
Data are means ± SE of 5–6 animals per group. Activities are expressed as units per gram wet weight. Citrate synthase, βHAD, and MCAD activities were determined directly in muscle homogenates, while CPT-1 activity was determined in isolated mitochondria and is expressed per gram wet weight based on recovery rates of citrate synthase (27).
* P < 0.05;
† P < 0.01 vs. standard diet–fed mice.