TABLE 2

Activity of enzymes of fatty acid utilization and oxidative capacity in standard diet–and fat-fed mice

5 weeks20 weeks
Standard dietFatStandard dietFat
Citrate synthase20.0 ± 1.625.0 ± 0.6*16.9 ± 1.926.5 ± 1.9
βHAD2.40 ± 0.183.19 ± 0.112.27 ± 0.283.82 ± 0.31
MCAD0.75 ± 0.051.12 ± 0.040.63 ± 0.101.40 ± 0.14
CPT-10.36 ± 0.050.49 ± 0.04*0.30 ± 0.020.47 ± 0.07*
  • Data are means ± SE of 5–6 animals per group. Activities are expressed as units per gram wet weight. Citrate synthase, βHAD, and MCAD activities were determined directly in muscle homogenates, while CPT-1 activity was determined in isolated mitochondria and is expressed per gram wet weight based on recovery rates of citrate synthase (27).

  • * P < 0.05;

  • P < 0.01 vs. standard diet–fed mice.